Any feedback?
Literature summary extracted from
- Cloning and characterization of a cyclohexanone monooxygenase gene from Arthrobacter sp. L661 (2008), Biotechnol. Bioprocess Eng., 13, 40-47.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.22 | gene chnB, genomic library screening, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. | - |
epsilon-caprolactone + NADP+ + H2O | - |
? |  |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. TK6 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. Phi1 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. L661 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. BP2 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. Phi2 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.22 | Arthrobacter sp. | A5HLX5 | gene chnB | - |
| 1.14.13.22 | Arthrobacter sp. | Q84H88 | gene chnB | - |
| 1.14.13.22 | Arthrobacter sp. BP2 | Q84H88 | gene chnB | - |
| 1.14.13.22 | Arthrobacter sp. L661 | A5HLX5 | gene chnB | - |
| 1.14.13.22 | Rhodococcus sp. | Q6RXW1 | gene chnB | - |
| 1.14.13.22 | Rhodococcus sp. | Q84H73 | - |
- |
| 1.14.13.22 | Rhodococcus sp. | Q84H76 | - |
- |
| 1.14.13.22 | Rhodococcus sp. Phi1 | Q84H73 | - |
- |
| 1.14.13.22 | Rhodococcus sp. Phi2 | Q84H76 | - |
- |
| 1.14.13.22 | Rhodococcus sp. TK6 | Q6RXW1 | gene chnB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.22 | recombinant His6-tagged enzyme 8.3fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arthrobacter sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.22 | additional information | the organism is capable of growth on cyclohexanone as a sole carbon source | Arthrobacter sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 24.75 | - |
purified His-tagged recombinant enzyme | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 24% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 7% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 24% of the activity with cyclohexanone | Rhodococcus sp. TK6 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. Phi1 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. L661 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,2-cyclohexandione + NADPH + H+ + O2 | 7% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 11% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. TK6 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. L661 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 11% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. Phi2 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 112% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 164% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 78% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. Phi1 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 112% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 1,4-cyclohexandione + NADPH + H+ + O2 | 164% of the activity with cyclohexanone | Rhodococcus sp. Phi2 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | 85% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | best substrate, 130% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | 159% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | 89% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | best substrate, 118% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | 81% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | best substrate, 172% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | 92% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | 98% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 3% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 92% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 65% of the activity with cyclohexanone | Rhodococcus sp. | dihydrofuran-2(3H)-one + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclodecanone + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclodecanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclodecanone + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclodecanone + NADPH + H+ + O2 | 6% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 108% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 166% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 73% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 80% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. TK6 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. TK6 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. Phi1 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. Phi1 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. L661 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. L661 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. BP2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. BP2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. Phi2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. Phi2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 17% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 23% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 42% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 37% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 42% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 48% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 65% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 95% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | additional information | no activity with 1,2-cyclohexandione | Rhodococcus sp. | ? | - |
? | |
| 1.14.13.22 | additional information | no activity with 1,3-cyclohexandione | Rhodococcus sp. | ? | - |
? | |
| 1.14.13.22 | additional information | no activity with cyclodecanone | Rhodococcus sp. | ? | - |
? | |
| 1.14.13.22 | additional information | substrate specificity of the recombinant His-tagged enzyme, overview | Arthrobacter sp. | ? | - |
? | |
| 1.14.13.22 | additional information | no activity with cyclodecanone | Rhodococcus sp. TK6 | ? | - |
? | |
| 1.14.13.22 | additional information | no activity with 1,3-cyclohexandione | Rhodococcus sp. Phi1 | ? | - |
? | |
| 1.14.13.22 | additional information | substrate specificity of the recombinant His-tagged enzyme, overview | Arthrobacter sp. L661 | ? | - |
? | |
| 1.14.13.22 | additional information | no activity with 1,2-cyclohexandione | Rhodococcus sp. Phi2 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.22 | CHMO | - |
Arthrobacter sp. |
| 1.14.13.22 | CHMO | - |
Rhodococcus sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 7 | - |
His-tagged recombinant enzyme | Arthrobacter sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 7 | 8.5 | 70% of maximal activity at pH 7.0 within this range, His-tagged recombinant enzyme | Arthrobacter sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 6 | 8 | purified recombinant enzyme, 70% remaining activity after 24 h at 4°C | Arthrobacter sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.22 | NADPH | - |
Arthrobacter sp. | |
| 1.14.13.22 | NADPH | - |
Rhodococcus sp. | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
