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Literature summary extracted from

  • Sakuma, M.; Kubota, S.
    Mouse AKR1E1 is an ortholog of pig liver NADPH dependent 1,5-anhydro-D-fructose reductase (2008), Biosci. Biotechnol. Biochem., 72, 872-876.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.263 DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) Mus musculus
1.1.1.292 AKR1E1 gene, cloning from liver RNA, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21 (DE3) Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.263 1.02
-
 1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus
1.1.1.292 1.02
-
 1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.263 34000
-
 x * 34000, about, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.263 1,5-anhydro-D-fructose + NADPH Mus musculus
-
 1,5-anhydro-D-glucitol + NADP+
-
 ?
1.1.1.292 1,5-anhydro-D-mannitol + NADP+ Mus musculus
-
 1,5-anhydro-D-fructose + NADPH
-
 r
1.1.1.292 1,5-anhydro-D-mannitol + NADP+ Mus musculus C57/BL6J
-
 1,5-anhydro-D-fructose + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.263 Mus musculus
-
-
-
1.1.1.292 Mus musculus Q9DCT1 C57BL/6J mice
-
1.1.1.292 Mus musculus C57/BL6J Q9DCT1 C57BL/6J mice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.263 recombinant enzyme 17.4fold from Escherichia coli strain BL21(DE3) by anion exchange and adsorption chromatography, and gel filtration Mus musculus
1.1.1.292 recombinant enzyme 17.6fold from Escherichia coli strain BL21 (DE3) by anion exchange and adsorption chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.263 3.01
-
 purified recombinant enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.263 1,5-anhydro-D-fructose + NADPH
-
 Mus musculus 1,5-anhydro-D-glucitol + NADP+
-
 ?
1.1.1.263 1,5-anhydro-D-fructose + NADPH preferred substrate Mus musculus 1,5-anhydro-D-glucitol + NADP+
-
 ?
1.1.1.263 2,3-butanedione + NADPH 76.4% of the activity with 1,5-anhydro-D-fructose Mus musculus ?
-
 ?
1.1.1.263 9,10-phenanthrenequinone + NADPH 18% of the activity with 1,5-anhydro-D-fructose Mus musculus ?
-
 ?
1.1.1.263 additional information substrate specificities of the recombinant enzyme for carbonyl compounds, overview Mus musculus ?
-
 ?
1.1.1.292 1,5-anhydro-D-mannitol + NADP+
-
 Mus musculus 1,5-anhydro-D-fructose + NADPH
-
 r
1.1.1.292 1,5-anhydro-D-mannitol + NADP+ 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction Mus musculus 1,5-anhydro-D-fructose + NADPH
-
 r
1.1.1.292 1,5-anhydro-D-mannitol + NADP+
-
 Mus musculus C57/BL6J 1,5-anhydro-D-fructose + NADPH
-
 r
1.1.1.292 1,5-anhydro-D-mannitol + NADP+ 1,5-anhydro-D-fructose is the preferred substrate for the reductase reaction Mus musculus C57/BL6J 1,5-anhydro-D-fructose + NADPH
-
 r
1.1.1.292 9,10-phenanthrenequinone + NADPH
-
 Mus musculus ? + NADP+
-
 ?
1.1.1.292 9,10-phenanthrenequinone + NADPH
-
 Mus musculus C57/BL6J ? + NADP+
-
 ?
1.1.1.292 butane-2,3-dione + NADPH
-
 Mus musculus acetoin + NADP+
-
 ?
1.1.1.292 butane-2,3-dione + NADPH
-
 Mus musculus C57/BL6J acetoin + NADP+
-
 ?
1.1.1.292 additional information D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview Mus musculus ?
-
 ?
1.1.1.292 additional information D-glucose, D-fructose, glucuronic acid, DL-glyceraldehyde, formaldehyde, and acetone are poor substrates, substrate specificity of the recombinant enzyme, overview Mus musculus C57/BL6J ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.263 ? x * 34000, about, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.263 AKR1E1
-
 Mus musculus
1.1.1.263 More the enzyme belongs to the aldo-keto reductase family 1 Mus musculus
1.1.1.263 NADPH dependent 1,5-anhydro-D-fructose reductase
-
 Mus musculus
1.1.1.292 AF reductase
-
 Mus musculus
1.1.1.292 AKR1E1
-
 Mus musculus
1.1.1.292 More the enzyme is a member of the aldo-keto reductase family 1 Mus musculus
1.1.1.292 NADPH dependent 1,5-anhydro-D-fructose reductase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.263 37
-
 assay at Mus musculus
1.1.1.292 37
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.292 9.3
-
 1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.263 7
-
 assay at Mus musculus
1.1.1.292 7
-
 assay at, reverse reaction Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.263 NADP+ dependent on Mus musculus
1.1.1.263 NADPH dependent on Mus musculus