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Literature summary extracted from

  • Kiryu, T.; Nakano, H.; Kiso, T.; Murakami, H.
    Purification and characterization of a carbohydrate: acceptor oxidoreductase from Paraconiothyrium sp. that produces lactobionic acid efficiently (2008), Biosci. Biotechnol. Biochem., 72, 833-841.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B3 0.03
-
 cellopentaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.06
-
 cellotriose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.06
-
 cellotetraose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.06
-
 cellohexaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.08
-
 cellobiose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.09
-
 xylobiose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.11
-
 lactose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.11
-
 cellotriose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.13
-
 cellobiose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.15
-
 cellopentaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.16
-
 cellohexaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.19
-
 lactose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.21
-
 xylobiose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.26
-
 cellotetraose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2 3 D-xylose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 4.9
-
 maltose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 6.7
-
 D-xylose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 11
-
 maltopentaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 11
-
 maltose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 12
-
 maltotetraose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 13
-
 maltohexaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 18
-
 maltotriose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 20
-
 D-glucose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 25
-
 D-glucose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 30
-
 maltohexaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 30
-
 maltotriose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 30
-
 maltotetraose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 32
-
 maltopentaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 47
-
 L-arabinose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 52
-
 6-deoxy-D-glucose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 61
-
 D-galactose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 75
-
 6-deoxy-D-glucose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 85
-
 L-arabinose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 91
-
 D-galactose O2 used as electron acceptor Paraconiothyrium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.B3 54000
-
 gel filtration, SDS-PAGE Paraconiothyrium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Paraconiothyrium sp.
-
 isolated as fungal strain KD-3 from soil, identified as Paraconiothyrium sp. according to 18S rDNA and ITS-5.8 S rDNA analysis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3 gel filtration, SDS-PAGE Paraconiothyrium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.B3 additional information
-
 enzyme purified and characterized, identified as a carbohydrate-acceptor oxidoreductase according to its molecular and structural properties and according to its specificity for electron donors and acceptors Paraconiothyrium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 6-deoxy-D-glucose + 2,6-dichlorophenol indophenol hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 6-deoxy-D-glucose + O2 hexoses as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 beta-D-glucose + 2,6-dichlorophenol indophenol DCIP, electron acceptor specificity determined Paraconiothyrium sp. D-glucono-1,5-lactone + ?
-
 ?
1.1.99.B3 beta-D-glucose + O2 kinetic parameters for various sugars shown, enzyme properties compared to other sugar oxidizing enzymes Paraconiothyrium sp. D-glucono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellobiose + 2,6-dichlorophenol indophenol disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 cellobiose + O2 disaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 cellohexaose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 cellopentaose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 cellotetraose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 cellotriose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-fructose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-galactose + 2,6-dichlorophenol indophenol hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-galactose + O2 hexoses as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-glucose + acceptor
-
 Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-mannose + O2 hexoses as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-xylobiose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-xylose + 2,6-dichlorophenol indophenol pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 D-xylose + O2 pentoses as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 L-arabinose + 2,6-dichlorophenol indophenol pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 L-arabinose + O2 pentoses as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 lactose + 2,6-dichlorophenol indophenol substrate specificity for alpha-lactose and beta-lactose determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 lactose + O2 substrate specificity for alpha-lactose and beta-lactose determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltohexaose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltopentaose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltose + 2,6-dichlorophenol indophenol disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltose + O2 disaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltotetraose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 maltotriose + O2 oligosaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 xylobiose + 2,6-dichlorophenol indophenol disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor Paraconiothyrium sp. ?
-
 ?
1.1.99.B3 xylobiose + O2 disaccharides as substrates determined Paraconiothyrium sp. ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.B3 0.25
-
 D-galactose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.35
-
 maltohexaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.45
-
 6-deoxy-D-glucose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.46
-
 D-glucose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.57
-
 maltohexaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.58
-
 D-xylose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.73
-
 L-arabinose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.76
-
 maltose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.81
-
 6-deoxy-D-glucose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 0.9
-
 maltopentaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.1
-
 L-arabinose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.1
-
 cellopentaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.1
-
 cellohexaose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.4
-
 cellotetraose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.5
-
 cellotriose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.5
-
 D-galactose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.7
-
 D-xylose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 1.8
-
 maltopentaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2
-
 maltose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2.1
-
 D-glucose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2.2
-
 maltotriose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2.3
-
 maltotriose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2.3
-
 maltotetraose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 2.6
-
 maltotetraose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 3
-
 xylobiose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 3.3
-
 xylobiose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 4.4
-
 lactose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 6.3
-
 lactose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 7.4
-
 cellobiose 2,6-dichlorophenol indophenol used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 7.5
-
 cellotriose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 9
-
 cellobiose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 9
-
 cellopentaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 10
-
 cellohexaose O2 used as electron acceptor Paraconiothyrium sp.
1.1.99.B3 11
-
 cellotetraose O2 used as electron acceptor Paraconiothyrium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.B3 5.5
-
 most active at pH 5.5 if O2 is used as electron acceptor Paraconiothyrium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.B3 2 7 stable in a pH range of 2.0-7.0 at 30°C Paraconiothyrium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.B3 FAD absorption spectra determined Paraconiothyrium sp.