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Literature summary extracted from

  • Kubota, T.; Shimono, J.; Kanameda, C.; Izumi, Y.
    The first thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase: cloning and overexpression of the gene from an extreme thermophile, Thermus thermophilus, and (2007), Biosci. Biotechnol. Biochem., 71, 3033-3040.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.29 overexpressed in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.29 0.03
-
 acetyl-CoA
-
 Thermus thermophilus
2.3.1.29 0.25
-
 glycine
-
 Thermus thermophilus
2.3.1.29 11.8
-
 L-alanine
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.29 43000
-
 SDS-PAGE Thermus thermophilus
2.3.1.29 85000
-
 native PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.29 Thermus thermophilus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.29 0.19
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.29 acetyl-CoA + glycine
-
 Thermus thermophilus CoA + L-2-amino-3-oxobutanoate
-
 ?
2.3.1.29 acetyl-CoA + L-alanine
-
 Thermus thermophilus CoA + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.29 dimer native PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.29 KBL enzyme is found to catalyze also the 7-keto-8-aminopelargnic acid (KAPA) synthase reaction Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.29 70
-
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.29 additional information
-
 enzyme shows high thermostability at 70°C for 1 h and a half-life of 1 h at 80°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.29 6
-
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.29 pyridoxal 5'-phosphate
-
 Thermus thermophilus