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Literature summary extracted from

  • Kawamura-Konishi, Y.; Tsuji, M.; Hatana, S.; Asanuma, M.; Kakuta, D.; Kawano, T.; Mukouyama, E.B.; Goto, H.; Suzuki, H.
    Purification, characterization, and molecular cloning of tyrosinase from Pholiota nameko (2007), Biosci. Biotechnol. Biochem., 71, 1752-1760.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1
-
 Pholiota nameko

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 30.6
-
 p-Cresol
-
 Pholiota nameko
1.14.18.1 119
-
 4-t-butylphenol
-
 Pholiota nameko
1.14.18.1 122
-
 dopamine
-
 Pholiota nameko
1.14.18.1 136
-
 4-t-butylcatechol
-
 Pholiota nameko
1.14.18.1 1240
-
 3-(3,4-dihydroxyphenyl) propionic acid
-
 Pholiota nameko
1.14.18.1 1930
-
 L-Dopa
-
 Pholiota nameko

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper enzyme contains 1.9 copper atoms per molecule Pholiota nameko

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 35000
-
 by gel filtration chromatography Pholiota nameko
1.14.18.1 42000
-
 by SDS-PAGE Pholiota nameko

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Pholiota nameko A7BHQ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Pholiota nameko

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit body
-
 Pholiota nameko
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 412
-
 purification step, Superdex 200 Pholiota nameko

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 3-(3,4-dihydroxyphenyl) propionic acid + 1/2 O2
-
 Pholiota nameko 3-(3,4-dihydroxyphenyl)propionic acid + H2O
-
 ?
1.14.18.1 4-t-butylphenol + O2 + AH2
-
 Pholiota nameko 4-t-butyl 1,2-benzoquinone + H2O + A
-
 ?
1.14.18.1 4-tert-butylcatechol + 1/2 O2
-
 Pholiota nameko 4-(tert-butyl)benzo-1,2-quinone + H2O
-
 ?
1.14.18.1 D-dopa + 1/2 O2
-
 Pholiota nameko D-dopaquinone + H2O
-
 ?
1.14.18.1 dopamine + 1/2 O2
-
 Pholiota nameko dopamine quinone + H2O
-
 ?
1.14.18.1 L-dopa + 1/2 O2
-
 Pholiota nameko L-dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2
-
 Pholiota nameko L-3,4-dihydroxyphenylalanine + H2O + A
-
 ?
1.14.18.1 p-cresol + O2 + AH2
-
 Pholiota nameko 4-methyl-o-quinone + H2O + A
-
 ?
1.14.18.1 tyramine + O2
-
 Pholiota nameko 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 monomer
-
 Pholiota nameko

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase
-
 Pholiota nameko
1.14.18.1 tyrosinase
-
 Pholiota nameko

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 13.2
-
 4-t-butylphenol
-
 Pholiota nameko
1.14.18.1 107
-
 p-Cresol
-
 Pholiota nameko
1.14.18.1 294
-
 4-t-butylcatechol
-
 Pholiota nameko
1.14.18.1 478
-
 L-Dopa
-
 Pholiota nameko
1.14.18.1 544
-
 3-(3,4-dihydroxyphenyl) propionic acid
-
 Pholiota nameko
1.14.18.1 1590
-
 dopamine
-
 Pholiota nameko