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Literature summary extracted from

  • Xie, S.-X.; Ogawa, J.; Shimizu, S.
    NAD+-Dependent (S)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2ol catalyzed by Nocardia fusca AKU 2123 (1999), Biosci. Biotechnol. Biochem., 63, 1721-1729.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B3 AgNO3 2 mM, no% residual activity Nocardia fusca AKU 2123
1.1.1.B3 CdCl2 2 mM, 6.6% residual activity Nocardia fusca AKU 2123
1.1.1.B3 EDTA 2 mM, 56% residual activity Nocardia fusca AKU 2123
1.1.1.B3 HgCl2 2 mM, no residual activity Nocardia fusca AKU 2123
1.1.1.B3 iodoacetate 2 mM, 67% residual activity Nocardia fusca AKU 2123
1.1.1.B3 o-phenanthroline 2 mM, 0.4% residual activity Nocardia fusca AKU 2123
1.1.1.B3 trypaflavine 1 mM, 7.7% residual activity Nocardia fusca AKU 2123

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B3 0.33
-
 (S)-2-hexanol 30°C, pH 7.5 Nocardia fusca AKU 2123
1.1.1.B3 1.6
-
 (S)-3-pentyn-2-ol 30°C, pH 7.5 Nocardia fusca AKU 2123
1.1.1.B3 2.5
-
 2-Hexanone 30°C, pH 7.5 Nocardia fusca AKU 2123

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B3 39000
-
 4 * 39000, SDS-PAGE Nocardia fusca AKU 2123
1.1.1.B3 150000
-
 gel filtration Nocardia fusca AKU 2123

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 Nocardia fusca AKU 2123
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B3
-
 Nocardia fusca AKU 2123

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B3 41.5
-
 30°C, pH 7.5 Nocardia fusca AKU 2123

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 (R)-1-phenylethanol + NAD+ 0.9% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 1-phenylethanone + NADH + H+
-
 r
1.1.1.B3 (R)-1-phenylethanol + NAD+ 21% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 1-phenylethanone + NADH + H+
-
 r
1.1.1.B3 (R)-2-butanol + NAD+ 6% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-butanone + NADH + H+
-
 r
1.1.1.B3 (R)-2-hexanol + NAD+ 30% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
 r
1.1.1.B3 (R)-2-pentanol + NAD+ 15% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-pentanone + NADH + H+
-
 r
1.1.1.B3 (R)-3-butyn-2-ol + NAD+ 0.5% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 but-3-yn-2-one + NADH + H+
-
 r
1.1.1.B3 (R)-3-pentyn-2-ol + NAD+ 5.6% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 pent-3-yn-2-one + NADH + H+
-
 r
1.1.1.B3 (S)-2-butanol + NAD+ 27% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-butanone + NADH + H+
-
 r
1.1.1.B3 (S)-2-hexanol + NAD+ 121% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
 r
1.1.1.B3 (S)-2-hexanol + NAD+ reaction is stereoselective Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
 r
1.1.1.B3 (S)-2-pentanol + NAD+ 91% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-pentanone + NADH + H+
-
 r
1.1.1.B3 (S)-3-butyn-2-ol + NAD+ 6.9% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 but-3-yn-2-one + NADH + H+
-
 r
1.1.1.B3 (S)-3-pentyn-2-ol + NAD+ 33% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 pent-3-yn-2-one + NADH + H+
-
 r
1.1.1.B3 1-hexanal + NADH + H+ 254% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 hexan-1-ol + NAD+
-
 r
1.1.1.B3 2-decanone + NADH + H+ 159% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-decanol + NAD+ reaction is stereoselective r
1.1.1.B3 2-heptanone + NADH + H+ 334% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-heptanol + NAD+ reaction is stereoselective r
1.1.1.B3 2-hexanone + NADH + H+ 225% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-hexanol + NAD+ reaction is stereoselective r
1.1.1.B3 2-octanone + NADH + H+ 223% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-octanol + NAD+ reaction is stereoselective r
1.1.1.B3 6-methyl-5-hepten-2-one + NADH + H+
-
 Nocardia fusca AKU 2123 (S)-6-methyl-5-hepten-2-ol + NAD+ 188% of the activity with (RS)-2-hexanol r
1.1.1.B3 ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
 Nocardia fusca AKU 2123 ethyl 4-chloro-3-oxobutanoate + NADH + H+ 38.1% of the activity with (RS)-2-hexanol r
1.1.1.B3 ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
 Nocardia fusca AKU 2123 ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
 r
1.1.1.B3 additional information primary alcohols are not accepted as substrate Nocardia fusca AKU 2123 ?
-
 ?
1.1.1.B3 n-valeraldehyde + NADH + H+ 84% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 ? + NAD+
-
 r
1.1.1.B3 pyruvic acid ethyl ester + NADH + H+ 225% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 ? + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.B3 tetramer 4 * 39000, SDS-PAGE Nocardia fusca AKU 2123

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B3 60
-
 oxidation reaction Nocardia fusca AKU 2123
1.1.1.B3 65
-
 reduction reaction Nocardia fusca AKU 2123

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.B3 40
-
 30 min, pH 7.0, 85% residual activity Nocardia fusca AKU 2123
1.1.1.B3 50
-
 30 min, pH 7.0, 50% residual activity Nocardia fusca AKU 2123

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.B3 5.5 6.5 reduction reaction Nocardia fusca AKU 2123
1.1.1.B3 8.5 9.5 oxidation reaction Nocardia fusca AKU 2123

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.B3 5.5 9.5 30 min, 30°C, more than 90% residual activity Nocardia fusca AKU 2123

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B3 additional information NADPH cannot substitute for NADH Nocardia fusca AKU 2123
1.1.1.B3 NAD+
-
 Nocardia fusca AKU 2123
1.1.1.B3 NADH
-
 Nocardia fusca AKU 2123