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Literature summary extracted from

  • Doeven, M.K.; van den Bogaart, G.; Krasnikov, V.; Poolman, B.
    Probing receptor-translocator interactions in the oligopeptide ABC transporter by fluorescence correlation spectroscopy (2008), Biophys. J., 94, 3956-3965.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.6 expression of C-terminal His6-tagged OppA, OppA I602C, and OppBCDF His6-tag on C-terminus of OppC in Lactococcus lactis Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.6 I602C OppA mutant, comparison to the wild-type enzyme in reconstitution in the membrane, substrate binding, and catalysis Lactococcus lactis
7.4.2.6 additional information OppA and OppBCDF are labeled with fluorescent probes, reconstituted into giant unilamellar vesicles, and the receptor-translocator interactions are investigated by fluorescence correlation spectroscopy, lateral mobility of OppA is reduced in OppBCDF-containing giant unilamellar vesicles distinguishing ligand-bound from ligand-free OppA, overview Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.6 membrane the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place Lactococcus lactis 16020
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.4.2.6 Mg2+ required Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.6 ATP + H2O + olipopeptide/out Lactococcus lactis the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place, mechanism studied in model membrane vesicles, overview ADP + phosphate + oligopeptide/in
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Organism

EC Number Organism UniProt Comment Textmining
7.4.2.6 Lactococcus lactis
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Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.6 recombinant His-tagged wild-type and mutant enzymes from Lactococcus lactis by nickel affinity chromatography Lactococcus lactis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
7.4.2.6 OppA and OppBCDF are reconstituted into giant unilamellar vesicles, overview Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.6 ATP + H2O + olipopeptide/out
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Lactococcus lactis ADP + phosphate + oligopeptide/in
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7.4.2.6 ATP + H2O + olipopeptide/out the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place, mechanism studied in model membrane vesicles, overview Lactococcus lactis ADP + phosphate + oligopeptide/in
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?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.6 oligopeptide ABC transporter
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Lactococcus lactis
7.4.2.6 oligopeptide transporter
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Lactococcus lactis
7.4.2.6 Opp
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Lactococcus lactis