EC Number | Cloned (Comment) | Organism |
---|---|---|
7.4.2.6 | expression of C-terminal His6-tagged OppA, OppA I602C, and OppBCDF His6-tag on C-terminus of OppC in Lactococcus lactis | Lactococcus lactis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.4.2.6 | I602C | OppA mutant, comparison to the wild-type enzyme in reconstitution in the membrane, substrate binding, and catalysis | Lactococcus lactis |
7.4.2.6 | additional information | OppA and OppBCDF are labeled with fluorescent probes, reconstituted into giant unilamellar vesicles, and the receptor-translocator interactions are investigated by fluorescence correlation spectroscopy, lateral mobility of OppA is reduced in OppBCDF-containing giant unilamellar vesicles distinguishing ligand-bound from ligand-free OppA, overview | Lactococcus lactis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.4.2.6 | membrane | the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place | Lactococcus lactis | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.4.2.6 | Mg2+ | required | Lactococcus lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.6 | ATP + H2O + olipopeptide/out | Lactococcus lactis | the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place, mechanism studied in model membrane vesicles, overview | ADP + phosphate + oligopeptide/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.4.2.6 | Lactococcus lactis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.4.2.6 | recombinant His-tagged wild-type and mutant enzymes from Lactococcus lactis by nickel affinity chromatography | Lactococcus lactis |
EC Number | Renatured (Comment) | Organism |
---|---|---|
7.4.2.6 | OppA and OppBCDF are reconstituted into giant unilamellar vesicles, overview | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.6 | ATP + H2O + olipopeptide/out | - |
Lactococcus lactis | ADP + phosphate + oligopeptide/in | - |
? | |
7.4.2.6 | ATP + H2O + olipopeptide/out | the extracytoplasmic lipid-anchored substrate-binding protein, or receptor, OppA delivers peptides to an integral membrane complex OppBCDF, or translocator, where, on ATP binding and hydrolysis, translocation across the membrane takes place, mechanism studied in model membrane vesicles, overview | Lactococcus lactis | ADP + phosphate + oligopeptide/in | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.4.2.6 | oligopeptide ABC transporter | - |
Lactococcus lactis |
7.4.2.6 | oligopeptide transporter | - |
Lactococcus lactis |
7.4.2.6 | Opp | - |
Lactococcus lactis |