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Literature summary extracted from

  • Kunii, D.; Shimoji, M.; Nakama, S.; Ikebe, M.; Hachiman, T.; Sato, I.; Tamaki, A.; Yamazaki, K.; Aniya, Y.
    Purification of liver serine protease which activates microsomal glutathione S-transferase: possible involvement of hepsin (2006), Biol. Pharm. Bull., 29, 868-874.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.106 CaCl2 43% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 dithiothreitol 94% inhibition at 10 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 E64 31% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 EDTA 8% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 leupeptin 96% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 MgCl2 21% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 N-alpha-p-tosyl-L-lysine chloromethyl ketone 12% inhibition at 0.1 mM, with t-butyloxycarbonylc-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 N-ethylmaleimide 25% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 N-tosyl-L-phenylalanine chloromethyl ketone 13% inhibition at 0.1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 NaN3 34% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus
3.4.21.106 ZnSO4 59% inhibition at 1 mM, with t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide as substrate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.106 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.106 19000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus
3.4.21.106 31000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus
3.4.21.106 41000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus
3.4.21.106 42000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus
3.4.21.106 55000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus
3.4.21.106 62000
-
 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.106 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.106 by gel filtration, 14880fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.106 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.106 microsomal glutathione S-transferase 1 + H2O hepsin seems to activate through microsomal glutathione S-transferase 1 dimer formation Rattus norvegicus active microsomal glutathione S-transferase
-
 ?
3.4.21.106 Suc-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O 2% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O 12% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O 100% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumaryl-7-amide + H2O 58% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Glu-Ala-Arg-4-methylcoumaryl-7-amide + H2O 41% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O 8% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O 25% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumaryl-7-amide + H2O 19% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O 13% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O 20% active with the purifed enzyme Rattus norvegicus ?
-
 ?
3.4.21.106 Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O 9% active with the purifed enzyme Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.106 ? 1 * 55000, 1 * 42000, 1 * 41000, under non-reducing conditions, SDS-PAGE. 1 * 31000, 1 * 19000, under reducing conditions, SDS-PAGE. 1 * 62000, MALDI-TOF mass spectrometry Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.106 type II transmembrane serine protease
-
 Rattus norvegicus