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Literature summary extracted from

  • Boselli, A.; Piubelli, L.; Molla, G.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
    Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity (2007), Biochimie, 89, 360-368.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 M213E mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 M213R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Q339E mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Q339N mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 R285A/D/K/Q inactive Rhodotorula toruloides
1.4.3.3 S335G mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 S335H mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 S335R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y223F mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y223S mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y238F mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y238R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y238S mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.22
-
 D-alanine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.4
-
 D-alanine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.4
-
 D-alanine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.4
-
 D-alanine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.5
-
 D-alanine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.6
-
 D-alanine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.7
-
 D-alanine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.8
-
 D-alanine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1
-
 cephalosporin C mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1
-
 cephalosporin C mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.1
-
 D-alanine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.1
-
 D-alanine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.3
-
 D-arginine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.3
-
 D-alanine mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.7
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.9
-
 cephalosporin C mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.9
-
 cephalosporin C mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2
-
 D-Aspartate mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2 3 D-arginine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.5
-
 D-arginine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.5
-
 cephalosporin C mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.8
-
 cephalosporin C mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.8
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 4.3
-
 cephalosporin C mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 5
-
 cephalosporin C wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 5.2
-
 D-Aspartate mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 5.5
-
 D-arginine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 5.5
-
 D-arginine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 6.7
-
 D-arginine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 8.7
-
 cephalosporin C mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 9.2
-
 D-arginine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 9.7
-
 D-arginine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 13
-
 cephalosporin C mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 13
-
 D-arginine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 15
-
 D-Aspartate mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 15
-
 D-Aspartate mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 17
-
 D-Aspartate mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 18
-
 D-alanine mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 18
-
 D-Aspartate wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 18
-
 D-arginine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 28
-
 cephalosporin C mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 29
-
 D-arginine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 30
-
 D-Aspartate mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 33
-
 D-Aspartate mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 40
-
 D-alanine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 53
-
 D-Aspartate mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 61
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 72
-
 D-Aspartate mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 98
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 150
-
 D-Aspartate mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 40000
-
 SDS-PAGE Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides P80324
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 phenyl-Sepharose column chromatography Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 104
-
 purified wild type enzyme, at 25°C Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-arginine + H2O + O2
-
 Rhodotorula toruloides 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-aspartate + H2O + O2
-
 Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 additional information the wild-type DAAO is mainly active on neutral D-amino acids, while basic D-amino acids are poor substrates and the acidic ones are virtually not oxidized Rhodotorula toruloides ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Rhodotorula toruloides
1.4.3.3 DAAO
-
 Rhodotorula toruloides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 0.09
-
 D-Aspartate mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.2
-
 D-arginine kcat less than 0.2/s, mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.26
-
 D-Aspartate mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.37
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.43
-
 D-arginine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.5
-
 D-Aspartate mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.5
-
 D-Aspartate wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.56
-
 D-Aspartate mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.63
-
 D-alanine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.67
-
 D-Aspartate mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.79
-
 D-alanine mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 0.9
-
 D-Aspartate mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 1.3
-
 cephalosporin C mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.1
-
 D-arginine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.5
-
 D-Aspartate mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 2.6
-
 D-arginine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.2
-
 D-Aspartate mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.2
-
 D-arginine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.6
-
 cephalosporin C mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.6
-
 D-arginine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.7
-
 D-alanine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 3.9
-
 D-Aspartate mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 4.4
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 6
-
 D-Aspartate mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 6.5
-
 D-arginine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 6.9
-
 D-alanine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 7
-
 cephalosporin C mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 7.3
-
 cephalosporin C mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 7.3
-
 D-arginine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 7.8
-
 cephalosporin C mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 8.6
-
 D-arginine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 9.2
-
 D-alanine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 10
-
 D-alanine mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 11
-
 D-arginine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 14
-
 D-arginine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 15
-
 D-alanine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 15
-
 cephalosporin C mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 17
-
 cephalosporin C mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 20
-
 D-arginine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 24
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 24
-
 cephalosporin C mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 25
-
 D-alanine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 25
-
 D-alanine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 26
-
 cephalosporin C mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 48
-
 D-alanine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 50
-
 cephalosporin C mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 67
-
 D-alanine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 72
-
 cephalosporin C wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 75
-
 D-alanine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.4.3.3 81
-
 D-alanine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD flavoprotein Rhodotorula toruloides