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Literature summary extracted from

  • Moore, R.L.; Powell, L.J.; Goodwin, D.C.
    The kinetic properties producing the perfunctory pH profiles of catalase-peroxidases (2008), Biochim. Biophys. Acta, 1784, 900-907.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) inhibition is pH-dependent. Lowest concentration where inhibition is observed is 1.2 mM at pH 4.5, 0.6 mM at pH 4.3, 0.3 mM at pH 4.0 Escherichia coli
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) acts as an inhibitor of peroxidase activity at lower pH (pH 3.8-4.5) Escherichia coli
1.11.1.21 H2O2 enzyme inhibition is observed at hydrogen peroxide concentrations greater than 1.0 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.032
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 23°C Escherichia coli
1.11.1.21 0.04
-
 o-Dianisidine pH 4.0, 23°C Escherichia coli
1.11.1.21 0.05
-
 o-Dianisidine pH 4.5, 23°C Escherichia coli
1.11.1.21 0.053
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 23°C Escherichia coli
1.11.1.21 0.06
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 23°C Escherichia coli
1.11.1.21 0.087
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 23°C Escherichia coli
1.11.1.21 0.09
-
 o-Dianisidine pH 5.0, 23°C Escherichia coli
1.11.1.21 0.09
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.5, 23°C Escherichia coli
1.11.1.21 0.11
-
 o-Dianisidine pH 5.5, 23°C Escherichia coli
1.11.1.21 0.12
-
 o-Dianisidine pH 6.0, 23°C Escherichia coli
1.11.1.21 1.1
-
 pyrogallol pH 4.5, 23°C Escherichia coli
1.11.1.21 1.1
-
 pyrogallol pH 4.0, 23°C Escherichia coli
1.11.1.21 1.3
-
 pyrogallol pH 5.0, 23°C Escherichia coli
1.11.1.21 1.5
-
 pyrogallol pH 5.5, 23°C Escherichia coli
1.11.1.21 1.6
-
 pyrogallol pH 6.0, 23°C Escherichia coli
1.11.1.21 3.8
-
 H2O2 pH 7.0, 23°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Escherichia coli
-
 bifunctional catalase-peroxidase KatG
-
1.11.1.21 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Escherichia coli O2 + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 H2O2
-
 Escherichia coli O2 + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Escherichia coli ? + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.6 11
-
 H2O2 presence of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 6.0 Escherichia coli
1.11.1.6 20
-
 H2O2 presence of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 5.5 Escherichia coli
1.11.1.6 24
-
 H2O2 presence of o-dianisidine, pH 4.0 Escherichia coli
1.11.1.6 27
-
 H2O2 presence of pyrogallol, pH 4.0 Escherichia coli
1.11.1.6 37
-
 H2O2 presence of o-dianisidine, pH 4.5 Escherichia coli
1.11.1.6 44
-
 H2O2 presence of pyrogallol, pH 4.5 Escherichia coli
1.11.1.6 55
-
 H2O2 presence of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 5.0 Escherichia coli
1.11.1.6 56
-
 H2O2 presence of pyrogallol, pH 5.0 Escherichia coli
1.11.1.6 58
-
 H2O2 presence of o-dianisidine, pH 5.0 Escherichia coli
1.11.1.6 64
-
 H2O2 presence of pyrogallol, pH 5.5 Escherichia coli
1.11.1.6 69
-
 H2O2 presence of o-dianisidine, pH 5.5 Escherichia coli
1.11.1.6 69
-
 H2O2 presence of pyrogallol, pH 6.0 Escherichia coli
1.11.1.6 71
-
 H2O2 presence of o-dianisidine, pH 6.0 Escherichia coli
1.11.1.6 92
-
 H2O2 presence of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.5 Escherichia coli
1.11.1.6 151
-
 H2O2 presence of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0 Escherichia coli
1.11.1.21 11
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 23°C Escherichia coli
1.11.1.21 20
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.5, 23°C Escherichia coli
1.11.1.21 24
-
 o-Dianisidine pH 4.0, 23°C Escherichia coli
1.11.1.21 27
-
 pyrogallol pH 4.0, 23°C Escherichia coli
1.11.1.21 37
-
 o-Dianisidine pH 4.5, 23°C Escherichia coli
1.11.1.21 44
-
 pyrogallol pH 4.5, 23°C Escherichia coli
1.11.1.21 55
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 23°C Escherichia coli
1.11.1.21 56
-
 pyrogallol pH 5.0, 23°C Escherichia coli
1.11.1.21 58
-
 o-Dianisidine pH 5.0, 23°C Escherichia coli
1.11.1.21 64
-
 pyrogallol pH 5.5, 23°C Escherichia coli
1.11.1.21 69
-
 o-Dianisidine pH 5.5, 23°C Escherichia coli
1.11.1.21 69
-
 pyrogallol pH 6.0, 23°C Escherichia coli
1.11.1.21 71
-
 o-Dianisidine pH 6.0, 23°C Escherichia coli
1.11.1.21 92
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 23°C Escherichia coli
1.11.1.21 151
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 23°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 additional information
-
 the apparent pH optimum is the intersection of the optimum for binding, 7.0, and the optimum for activity, 5.8 Escherichia coli
1.11.1.21 4.5
-
 peroxidase pH optimum Escherichia coli
1.11.1.21 6.5
-
 catalase pH optimum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Escherichia coli