Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Usselman, R.J.; Fielding, A.J.; Frerman, F.E.; Watmough, N.J.; Eaton, G.R.; Eaton, S.S.
    Impact of mutations on the midpoint potential of the [4Fe-4S]+1,+2 cluster and on catalytic activity in electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) (2008), Biochemistry, 47, 92-100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.5.1 expressed in Escherichia coli C43 cells Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
1.5.5.1 T525A the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides
1.5.5.1 Y501F the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides
1.5.5.1 Y501F/T525A the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.1 60000
-
 SDS-PAGE Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.1 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.1 Q-Sepharose colmn chromatography and Source 15Q column chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.1 reduced electron-transferring flavoprotein + ubiquinone-2
-
 Cereibacter sphaeroides electron-transferring flavoprotein + ubiquinol-2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.5.1 electron transfer flavoprotein-ubiquinone oxidoreductase
-
 Cereibacter sphaeroides
1.5.5.1 ETF-QO
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.5.1 4Fe-4S-center iron-sulfur flavoprotein that contains a single [4Fe-4S]2+,1+ Cereibacter sphaeroides
1.5.5.1 FAD contains one FAD Cereibacter sphaeroides