EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.3.1 | mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) | Bacillus sp. (in: Bacteria) |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.3.1 | K265A | only traces of activity | Bacillus sp. (in: Bacteria) |
1.5.3.1 | K265M | only traces of activity | Bacillus sp. (in: Bacteria) |
1.5.3.1 | K265Q | only traces of activity | Bacillus sp. (in: Bacteria) |
1.5.3.1 | K265R | less than 30% of covalently bound FAD, strongly reduced turnover | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.1 | methylthioacetate | competitive | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 44000 | - |
1 * 44000 | Bacillus sp. (in: Bacteria) |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + O2 + H2O | Bacillus sp. (in: Bacteria) | - |
formaldehyde + glycine + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.3.1 | Bacillus sp. (in: Bacteria) | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.3.1 | recombinant proteins using His-tag | Bacillus sp. (in: Bacteria) |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 | mechanism suggested | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + O2 + H2O | - |
Bacillus sp. (in: Bacteria) | formaldehyde + glycine + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.3.1 | monomer | 1 * 44000 | Bacillus sp. (in: Bacteria) |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.3.1 | monomeric sarcosine oxidase | - |
Bacillus sp. (in: Bacteria) |
1.5.3.1 | MSOX | - |
Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 0.0049 | - |
sarcosine | K265Q mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 0.0066 | - |
sarcosine | K265A mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 0.0076 | - |
sarcosine | K265M mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 0.272 | - |
sarcosine | K265R mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1.5.3.1 | 45.8 | - |
sarcosine | native protein, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.1 | FAD | covalently bound | Bacillus sp. (in: Bacteria) |