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Literature summary extracted from

  • Jiang, W.; Saleh, L.; Barr, E.W.; Xie, J.; Gardner, M.M.; Krebs, C.; Bollinger, J.M.
    Branched activation- and catalysis-specific pathways for electron relay to the manganese/iron cofactor in ribonucleotide reductase from Chlamydia trachomatis (2008), Biochemistry, 47, 8477-8484.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 O2 activates the MnIV/FeIII cofactor, overview Chlamydia trachomatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 overexpression of wild-type and mutant R2 subunits in Escherichia coli strain BL21(DE3) Chlamydia trachomatis

Protein Variants

EC Number Protein Variants Comment Organism
1.17.4.1 W51F site-directed mutagenesis, the decay of the Mn(IV)/Fe(IV) intermediate is slightly affected Chlamydia trachomatis
1.17.4.1 Y222F the substitution by site-directed mutagenesis retards the intrinsic decay of the Mn(IV)/Fe(IV) intermediate by about 10fold and diminishes the ability of ascorbate to accelerate the decay by about 65fold but has no detectable effect on the catalytic activity of the Mn(IV)/Fe(III)-R2 product Chlamydia trachomatis
1.17.4.1 Y338F site-directed mutagenesis, substitution of Y338, the cognate of the subunit interfacial R2 residue in the R1 S R2 PCET pathway of the conventional class I RNRs, has almost no effect on decay of the Mn(IV)/Fe(IV) intermediate but abolishes catalytic activity Chlamydia trachomatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for Mn(IV) in place of the Y• for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active Mn(IV)/Fe(III) state, the reduction step in this sequence is mediated by residue Y222, overview Chlamydia trachomatis
1.17.4.1 Manganese the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for Mn(IV) in place of the Y• for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active Mn(IV)/Fe(III) state, the reduction step in this sequence is mediated by residue Y222, overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 CDP + thioredoxin Chlamydia trachomatis
-
 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Chlamydia trachomatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
 Chlamydia trachomatis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 RNR
-
 Chlamydia trachomatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Chlamydia trachomatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.6
-
 assay at Chlamydia trachomatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 manganese-iron cofactor the class Ic RNR from Chlamydia trachomatis uses a MnIV/FeIII cofactor, with high specificity for MnIV in place of the tyrosyl radical for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active MnIV/FeIII state, the reduction step in this sequence is mediated by residue Y222, overview Chlamydia trachomatis
1.17.4.1 thioredoxin
-
 Chlamydia trachomatis