EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.30 | expressed in Escherichia coli BL21 cells | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.30 | 0.008 | - |
FMN | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
1.5.1.30 | 0.02 | - |
NADPH | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
1.5.1.30 | 0.072 | - |
FMN | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
1.5.1.30 | 0.31 | - |
NADPH | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.30 | 26300 | - |
2 * 26300, gel filtration | Vibrio harveyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.30 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.30 | the flavin reductase-luciferase fusion protein is purified using DEAE-cellulose DE-52 column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.30 | FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.30 | dimer | 2 * 26300, gel filtration | Vibrio harveyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.30 | FRP | - |
Vibrio harveyi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.30 | 11.7 | - |
FMN | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
1.5.1.30 | 37.5 | - |
FMN | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.30 | FMN | - |
Vibrio harveyi | |
1.5.1.30 | NADPH | - |
Vibrio harveyi |