Literature summary extracted from
Echalier, A.; Brittain, T.; Wright, J.; Boycheva, S.; Mortuza, G.B.; Fueloep, V.; Watmough, N.J.
Redox-linked structural changes associated with the formation of a catalytically competent form of the diheme cytochrome c peroxidase from Pseudomonas aeruginosa (2008), Biochemistry, 47, 1947-1956.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.11.1.5 |
expressed in Escherichia coli JM109 (DE3) cells |
Pseudomonas aeruginosa |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.11.1.5 |
with 24% PEG 600, 0.2 M imidazole malate pH 5.5, 20 mM dithiothreitol |
Pseudomonas aeruginosa |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.11.1.5 |
Ca2+ |
a single, tightly bound, Ca2+ ion at the domain interface of both the fully oxidized and mixed-valence forms of the enzyme is absolutely required for catalytic activity, reduction of the electron-transferring (high-potential) heme in the presence of Ca2+ ions triggers substantial structural rearrangements around the active-site (low-potential) heme to allow substrate binding and catalysis |
Pseudomonas aeruginosa |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.11.1.5 |
Pseudomonas aeruginosa |
P14532 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.11.1.5 |
S-Sepharose column chromatography and Superdex-75 gel filtration |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.11.1.5 |
ferrocytochrome c + H2O2 |
- |
Pseudomonas aeruginosa |
ferricytochrome c + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.11.1.5 |
CCP |
- |
Pseudomonas aeruginosa |
1.11.1.5 |
cytochrome c peroxidase |
- |
Pseudomonas aeruginosa |