BRENDA - Enzyme Database

Determinants of substrate specificity for saccharopine dehydrogenase from Saccharomyces cerevisiae

Xu, H.; West, A.H.; Cook, P.F.; Biochemistry 46, 7625-7636 (2007)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.1.7
2',3'-cyclic NADP
-
Saccharomyces cerevisiae
1.5.1.7
2',3'-cyclic NADP+
-
Saccharomyces cerevisiae
1.5.1.7
adenosine
-
Saccharomyces cerevisiae
1.5.1.7
adipate
-
Saccharomyces cerevisiae
1.5.1.7
ADP
-
Saccharomyces cerevisiae
1.5.1.7
ADP-ribose
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoadipate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoglutarate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoisovalerate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketomalonate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketopimelate
-
Saccharomyces cerevisiae
1.5.1.7
AMP
-
Saccharomyces cerevisiae
1.5.1.7
D-Lysine
-
Saccharomyces cerevisiae
1.5.1.7
Glutarate
-
Saccharomyces cerevisiae
1.5.1.7
L-arginine
-
Saccharomyces cerevisiae
1.5.1.7
L-glutamine
-
Saccharomyces cerevisiae
1.5.1.7
L-isoleucine
-
Saccharomyces cerevisiae
1.5.1.7
L-leucine
dead-end inhibition by L-leucine is uncompetitive versus NADPH
Saccharomyces cerevisiae
1.5.1.7
L-lysine
-
Saccharomyces cerevisiae
1.5.1.7
L-methionine
-
Saccharomyces cerevisiae
1.5.1.7
L-norvaline
-
Saccharomyces cerevisiae
1.5.1.7
L-ornithine
-
Saccharomyces cerevisiae
1.5.1.7
L-valine
-
Saccharomyces cerevisiae
1.5.1.7
malonate
-
Saccharomyces cerevisiae
1.5.1.7
additional information
not inhibited by L-asparagine at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
NAD+
-
Saccharomyces cerevisiae
1.5.1.7
NADH
-
Saccharomyces cerevisiae
1.5.1.7
NADP+
NADP+ is competitive versus NADPH when NADPH serves as the coenzyme
Saccharomyces cerevisiae
1.5.1.7
NMN
-
Saccharomyces cerevisiae
1.5.1.7
oxalate
-
Saccharomyces cerevisiae
1.5.1.7
oxaloacetate
-
Saccharomyces cerevisiae
1.5.1.7
oxalylglycine
-
Saccharomyces cerevisiae
1.5.1.7
pyridine 2,3-dicarboxylate
-
Saccharomyces cerevisiae
1.5.1.7
Pyridine 2,4-dicarboxylate
-
Saccharomyces cerevisiae
1.5.1.7
succinate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.7
0.11
-
2-oxoglutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
4.1
-
pyruvate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.3
-
alpha-ketoadipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
6.4
-
glyoxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
alpha-ketomalonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
94
-
alpha-ketovalerate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
153
-
alpha-Ketobutyrate
at pH 7.0
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.7
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.7
L-lysine + alpha-ketoadipate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketobutyrate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketomalonate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketovalerate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + glyoxylate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + pyruvate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
additional information
3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, and thionicotinamide adenine dinucleotide can serve as a substrate in the oxidative deamination reaction, as can glyoxylate, pyruvate, alpha-ketobutyrate, alpha-ketovalerate, alpha-ketomalonate, and alpha-ketoadipate in the reverse reaction
685171
Saccharomyces cerevisiae
?
-
-
-
-
1.5.1.7
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
the enzyme is specific for L-lysine
685171
Saccharomyces cerevisiae
L-lysine + 2-oxoglutarate + NADH + H+
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.7
NAD+
-
Saccharomyces cerevisiae
1.5.1.7
NADH
-
Saccharomyces cerevisiae
1.5.1.7
NADPH
NADPH can substitute for NADH
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5.1.7
0.018
-
NADH
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.055
-
AMP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.093
-
ADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.1
-
oxalylglycine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.125
-
L-leucine
at pH 6.0
Saccharomyces cerevisiae
1.5.1.7
0.29
-
ADP-ribose
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.42
-
adenosine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.6
-
alpha-ketoglutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1
-
Glutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.1
-
L-lysine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.1
-
Pyridine 2,4-dicarboxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.13
-
L-norvaline
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.2
-
NADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.5
-
2',3'-cyclic NADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.56
-
NAD+
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
3.12
-
L-methionine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
3.5
-
NADP+
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5
-
L-ornithine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.3
-
alpha-ketoadipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.5
-
D-Lysine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.6
-
L-leucine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
6.4
-
oxaloacetate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
7.2
-
NMN
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
16
-
L-glutamine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
16.4
-
L-arginine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
18
-
alpha-ketopimelate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
18.3
-
pyridine 2,3-dicarboxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
21
-
succinate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
22
-
L-isoleucine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
alpha-ketomalonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
malonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
36
-
alpha-ketoisovalerate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
36
-
oxalate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
50
-
adipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
140
-
L-valine
at pH 7.0
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.7
NAD+
-
Saccharomyces cerevisiae
1.5.1.7
NADH
-
Saccharomyces cerevisiae
1.5.1.7
NADPH
NADPH can substitute for NADH
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.1.7
2',3'-cyclic NADP
-
Saccharomyces cerevisiae
1.5.1.7
2',3'-cyclic NADP+
-
Saccharomyces cerevisiae
1.5.1.7
adenosine
-
Saccharomyces cerevisiae
1.5.1.7
adipate
-
Saccharomyces cerevisiae
1.5.1.7
ADP
-
Saccharomyces cerevisiae
1.5.1.7
ADP-ribose
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoadipate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoglutarate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketoisovalerate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketomalonate
-
Saccharomyces cerevisiae
1.5.1.7
alpha-ketopimelate
-
Saccharomyces cerevisiae
1.5.1.7
AMP
-
Saccharomyces cerevisiae
1.5.1.7
D-Lysine
-
Saccharomyces cerevisiae
1.5.1.7
Glutarate
-
Saccharomyces cerevisiae
1.5.1.7
L-arginine
-
Saccharomyces cerevisiae
1.5.1.7
L-glutamine
-
Saccharomyces cerevisiae
1.5.1.7
L-isoleucine
-
Saccharomyces cerevisiae
1.5.1.7
L-leucine
dead-end inhibition by L-leucine is uncompetitive versus NADPH
Saccharomyces cerevisiae
1.5.1.7
L-lysine
-
Saccharomyces cerevisiae
1.5.1.7
L-methionine
-
Saccharomyces cerevisiae
1.5.1.7
L-norvaline
-
Saccharomyces cerevisiae
1.5.1.7
L-ornithine
-
Saccharomyces cerevisiae
1.5.1.7
L-valine
-
Saccharomyces cerevisiae
1.5.1.7
malonate
-
Saccharomyces cerevisiae
1.5.1.7
additional information
not inhibited by L-asparagine at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
NAD+
-
Saccharomyces cerevisiae
1.5.1.7
NADH
-
Saccharomyces cerevisiae
1.5.1.7
NADP+
NADP+ is competitive versus NADPH when NADPH serves as the coenzyme
Saccharomyces cerevisiae
1.5.1.7
NMN
-
Saccharomyces cerevisiae
1.5.1.7
oxalate
-
Saccharomyces cerevisiae
1.5.1.7
oxaloacetate
-
Saccharomyces cerevisiae
1.5.1.7
oxalylglycine
-
Saccharomyces cerevisiae
1.5.1.7
pyridine 2,3-dicarboxylate
-
Saccharomyces cerevisiae
1.5.1.7
Pyridine 2,4-dicarboxylate
-
Saccharomyces cerevisiae
1.5.1.7
succinate
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5.1.7
0.018
-
NADH
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.055
-
AMP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.093
-
ADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.1
-
oxalylglycine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.125
-
L-leucine
at pH 6.0
Saccharomyces cerevisiae
1.5.1.7
0.29
-
ADP-ribose
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.42
-
adenosine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
0.6
-
alpha-ketoglutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1
-
Glutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.1
-
L-lysine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.1
-
Pyridine 2,4-dicarboxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.13
-
L-norvaline
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.2
-
NADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.5
-
2',3'-cyclic NADP
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
1.56
-
NAD+
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
3.12
-
L-methionine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
3.5
-
NADP+
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5
-
L-ornithine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.3
-
alpha-ketoadipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.5
-
D-Lysine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.6
-
L-leucine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
6.4
-
oxaloacetate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
7.2
-
NMN
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
16
-
L-glutamine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
16.4
-
L-arginine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
18
-
alpha-ketopimelate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
18.3
-
pyridine 2,3-dicarboxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
21
-
succinate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
22
-
L-isoleucine
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
alpha-ketomalonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
malonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
36
-
alpha-ketoisovalerate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
36
-
oxalate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
50
-
adipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
140
-
L-valine
at pH 7.0
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.7
0.11
-
2-oxoglutarate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
4.1
-
pyruvate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
5.3
-
alpha-ketoadipate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
6.4
-
glyoxylate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
24
-
alpha-ketomalonate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
94
-
alpha-ketovalerate
at pH 7.0
Saccharomyces cerevisiae
1.5.1.7
153
-
alpha-Ketobutyrate
at pH 7.0
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.7
L-lysine + alpha-ketoadipate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketobutyrate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketomalonate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + alpha-ketovalerate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + glyoxylate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
L-lysine + pyruvate + NADH
-
685171
Saccharomyces cerevisiae
?
-
-
-
?
1.5.1.7
additional information
3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, and thionicotinamide adenine dinucleotide can serve as a substrate in the oxidative deamination reaction, as can glyoxylate, pyruvate, alpha-ketobutyrate, alpha-ketovalerate, alpha-ketomalonate, and alpha-ketoadipate in the reverse reaction
685171
Saccharomyces cerevisiae
?
-
-
-
-
1.5.1.7
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
the enzyme is specific for L-lysine
685171
Saccharomyces cerevisiae
L-lysine + 2-oxoglutarate + NADH + H+
-
-
-
r