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Literature summary extracted from

  • Hegde, S.S.; Chandler, J.; Vetting, M.W.; Yu, M.; Blanchard, J.S.
    Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase (2007), Biochemistry, 46, 7187-7195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 expressed in Escherichia coli, strains Nova Blue and BL21(DE3), plasmid pET-28a Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.57 three-dimensional crystal structure of SSAT with bound bisubstrate inhibitor, solved at 2.3 A resolution, hanging drop method, data and refinement statistics Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.57 N1-spermine-acetyl-CoA bisubstrate analogue, bisubstrate inhibition patterns determined, linear, competitive inhibition against sperimidine and acetyl-CoA determined Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 additional information
-
additional information no detectable activity for putrescine and cadaverine Homo sapiens
2.3.1.57 0.0038
-
acetyl-CoA measured at fixed saturating concentration of spermidine Homo sapiens
2.3.1.57 0.0057
-
spermine measured at fixed saturating concentrations of acetyl-CoA Homo sapiens
2.3.1.57 0.022
-
spermidine measured at fixed saturating concentrations of acetyl-CoA Homo sapiens
2.3.1.57 0.107
-
1,3-diaminopropane measured at fixed saturating concentrations of acetyl-CoA Homo sapiens
2.3.1.57 0.194
-
diethylenetriamine measured at fixed saturating concentrations of acetyl-CoA Homo sapiens
2.3.1.57 0.196
-
ethylenediamine measured at fixed saturating concentrations of acetyl-CoA Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 acetyl-CoA + spermidine Homo sapiens
-
CoA + N1-acetylspermidine
-
?
2.3.1.57 acetyl-CoA + spermine Homo sapiens N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells CoA + N1-acetylspermine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Homo sapiens P21673 hSSAT gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 gel filtration, recombinant protein, all purification procedures at 4°C Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.57 commercial preparation cDNA clone of human SSAT Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.57 additional information
-
kinetic and mechanistic characterization, determination of bisubstrate inhibition patterns, three-dimensional structure of the recombinant human SSAT protein with bound bisubstrate inhibitor, acid/base assisted reaction catalyzed by SSAT involves a ternary complex, proposed on the base of kinetic properties, pH dependence and structure analysis Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + 1,3-diaminopropane
-
Homo sapiens CoA + N1-acetyl-1,3-diaminopropane
-
?
2.3.1.57 acetyl-CoA + diethylenetriamine
-
Homo sapiens CoA + N1-acetyl-diethylenetriamine
-
?
2.3.1.57 acetyl-CoA + ethylenediamine
-
Homo sapiens CoA + N1-acetyl-ethylenediamine
-
?
2.3.1.57 acetyl-CoA + spermidine
-
Homo sapiens CoA + N1-acetylspermidine
-
?
2.3.1.57 acetyl-CoA + spermine N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells Homo sapiens CoA + N1-acetylspermine
-
?
2.3.1.57 acetyl-CoA + spermine N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells, chemical and kinetic mechanism for acetyl transfer activity by recombinant human SSAT protein proposed Homo sapiens CoA + N1-acetylspermine
-
?
2.3.1.57 chloroacetyl-CoA + spermine N1-chloroacetylation of spermine performed by hSSAT protein, pH 7.5 and 2 microM recombinant human SSAT protein at room temperature for one hour, identity of the product confirmed by mass spectrometry Homo sapiens N1-spermine-acetyl-CoA + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 SSAT
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.57 8
-
determined by pH activity profile Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.57 6.7 9.1 activity monitored every 0.3 pH units, bell shaped pH activity profile, depending on ionization state of two groups with apparent pKa values of 7.27 and 8.87 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.57 0.006
-
N1-spermine-acetyl-CoA bisubstrate analogue, linear, competitive inhibition, structure of hSSAT with bound N1-spermine-acetyl-CoA determined Homo sapiens