EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | - |
Mus musculus |
1.8.1.9 | expression in Escherichia coli | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.1.9 | hanging drop diffusion method at 20°C, X-ray crystal structure of DmTR at 2.4 A resolution in which the enzyme was truncated to remove the C-terminal tripeptide sequence Cys-Cys-Ser | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.9 | additional information | the truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH | Mus musculus |
1.8.1.9 | additional information | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.032 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence SCCS | Mus musculus | |
1.8.1.9 | 0.0676 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
1.8.1.9 | 0.123 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCCG | Mus musculus | |
1.8.1.9 | 0.141 | - |
thioredoxin disulfide | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
1.8.1.9 | 0.17 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
1.8.1.9 | 0.47 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
1.8.1.9 | 0.92 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid | Drosophila melanogaster | |
1.8.1.9 | 2.72 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.1.9 | mitochondrion | - |
Mus musculus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | additional information | DmTR shows high catalytic activity without the presence of a selenocysteine | Drosophila melanogaster | |
1.8.1.9 | additional information | DmTR shows high catalytic activity without the presence of a selenocysteine | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Drosophila melanogaster | P91938 | - |
- |
1.8.1.9 | Mus musculus | Q8BTW3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | - |
Drosophila melanogaster |
1.8.1.9 | - |
Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Drosophila melanogaster | thioredoxin + NADP+ | - |
? | |
1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Mus musculus | thioredoxin + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | DmTR | - |
Drosophila melanogaster |
1.8.1.9 | mTR3 | - |
Mus musculus |
1.8.1.9 | thioredoxin reductase | - |
Drosophila melanogaster |
1.8.1.9 | thioredoxin reductase | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.083 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence SCCS | Mus musculus | |
1.8.1.9 | 0.13 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCCG | Mus musculus | |
1.8.1.9 | 1.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
1.8.1.9 | 2.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid | Drosophila melanogaster | |
1.8.1.9 | 5.8 | - |
thioredoxin disulfide | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
1.8.1.9 | 15.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid | Mus musculus | |
1.8.1.9 | 20.83 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
1.8.1.9 | 37 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | NADPH | - |
Drosophila melanogaster | |
1.8.1.9 | NADPH | - |
Mus musculus |