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Literature summary extracted from

  • Fagan, R.L.; Jensen, K.F.; Bjoernberg, O.; Palfey, B.A.
    Mechanism of flavin reduction in the class 1A dihydroorotate dehydrogenase from Lactococcus lactis (2007), Biochemistry, 46, 4028-4036.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.1 expressed in SO6645 cells Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.98.1 C130A the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau Lactococcus lactis
1.3.98.1 C130S the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 ammonium sulfate precipitation and Q-Sepharose column chromatography Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 L-dihydroorotate + fumarate
-
 Lactococcus lactis orotate + succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 class 1A DHOD
-
 Lactococcus lactis
1.3.98.1 class 1A dihydroorotate dehydrogenase
-
 Lactococcus lactis
1.3.98.1 DHODH
-
 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.98.1 FMN
-
 Lactococcus lactis