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Literature summary extracted from

  • Davies, A.M.; Tata, R.; Beavil, R.L.; Sutton, B.J.; Brown, P.R.
    l-Methionine sulfoximine, but not phosphinothricin, is a substrate for an acetyltransferase (gene PA4866) from Pseudomonas aeruginosa: structural and functional studies (2007), Biochemistry, 46, 1829-1839.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.183 into the vector pET24a for expression in Escherichia coli BL21DE3 cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.183 a high-resolution, 1.55 A, crystal structure of pita in complex with L-methionine sulfoximine is solved Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.183 Depp4 mutant, in which pita is inactivated through an in-frame deletion Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.183 0.03
-
acetyl-CoA apparent KM Pseudomonas aeruginosa
2.3.1.183 1.3
-
L-methionine sulfoximine apparent KM Pseudomonas aeruginosa
2.3.1.183 1.3
-
L-methionine sulfone apparent KM Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.183 Pseudomonas aeruginosa Q9HUU7 putative uncharacterized protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.183
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.183 acetyl-CoA + L-methionine sulfone
-
Pseudomonas aeruginosa ?
-
?
2.3.1.183 acetyl-CoA + L-methionine sulfoximine enzyme has no activity towards phosphinothricin Pseudomonas aeruginosa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.183 phosphinothricin acetyltransferase
-
Pseudomonas aeruginosa
2.3.1.183 PitA
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.183 37
-
activity assay Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.183 505
-
L-methionine sulfoximine apparent Kcat Pseudomonas aeruginosa
2.3.1.183 610
-
L-methionine sulfone apparent Kcat Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.183 7.2
-
activity assay Pseudomonas aeruginosa