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Literature summary extracted from

  • Manieri, W.; Moore, M.E.; Soellner, M.B.; Tsang, P.; Caperelli, C.A.
    Human glycinamide ribonucleotide transformylase: active site mutants as mechanistic probes (2007), Biochemistry, 46, 156-163.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.2.2 medicine glycinamide ribonucleotide transformylase is a validated target for cancer chemotherapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.2 D144E catalytically inactive mutant Homo sapiens
2.1.2.2 D144H catalytically inactive mutant Homo sapiens
2.1.2.2 H108K catalytically inactive mutant Homo sapiens
2.1.2.2 H108R catalytically inactive mutant Homo sapiens
2.1.2.2 K170R catalytically active mutant Homo sapiens
2.1.2.2 N106D catalytically inactive mutant Homo sapiens
2.1.2.2 N106H catalytically inactive mutant Homo sapiens
2.1.2.2 N106Q catalytically active mutant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.0009
-
10-formyl-5,8-dideazafolate wild type enzyme Homo sapiens
2.1.2.2 0.0011
-
beta-glycinamide ribonucleotide wild type enzyme Homo sapiens
2.1.2.2 0.0015
-
10-formyl-5,8-dideazafolate mutant K170R Homo sapiens
2.1.2.2 0.0018
-
beta-glycinamide ribonucleotide mutant K170R Homo sapiens
2.1.2.2 0.0196
-
10-formyl-5,8-dideazafolate mutant N106Q Homo sapiens
2.1.2.2 0.0295
-
beta-glycinamide ribonucleotide mutant N106Q Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 108000
-
human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2 using a NiHisTrap HP column, the affinity tag is removed by cleavage with TEV protease Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyl-5,8-dideazafolate + N1-(5-phospho-D-ribosyl)glycinamide
-
Homo sapiens ?
-
?
2.1.2.2 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
-
Homo sapiens tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
-
?
2.1.2.2 beta-glycinamide ribonucleotide + 10-formyltetrahydrofolate
-
Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.2 GART
-
Homo sapiens
2.1.2.2 glycinamide ribonucleotide transformylase
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.2 7.5
-
beta-glycinamide ribonucleotide wild type enzyme Homo sapiens
2.1.2.2 7.7
-
beta-glycinamide ribonucleotide mutant K170R Homo sapiens
2.1.2.2 14
-
beta-glycinamide ribonucleotide mutant N106Q Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.2 8
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.2 5 11
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.2 folate
-
Homo sapiens