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Literature summary extracted from

  • Zheng, J.; Domsic, J.F.; Cabelli, D.; McKenna, R.; Silverman, D.N.
    Structural and kinetic study of differences between human and Escherichia coli manganese superoxide dismutases (2007), Biochemistry, 46, 14830-14837.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sod, expression of wild-type and mutant soluble enzymes in Escherichia coli strain QC774, that lacks the genes encoding endogeneous FeSOD, SodB-, and MnSOD, SodA- Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.15.1.1 mutant enzymes F66A and F66L, hanging drop vapor diffusion method, 0.005 ml of enzyme solution are mixed with 0.005 ml of precipitant solution containing 2.5 M ammonium sulfate, 100 mM imidazole, and 100 mM malic acid, pH 8.5, equilibration against 1 ml of precipitant solution, 1 week, room temperature, X-ray diffraction structure determination and analysis at 2.2 A and 2.3 A resolution, respectively Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 F66A site-directed mutagenesis, alteration of the active site surrounding, the mutant is 3fold less sensitive to product inhibition compared to the wild-type enzyme Homo sapiens
1.15.1.1 F66L site-directed mutagenesis, alteration of the active site surrounding, the mutant shows residual product inhibition with formation of a peroxide-inhibited enzyme and increased catalytic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 additional information the conserved, active-site residue Tyr34 mediates product inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.1 additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ a Mn-SOD Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2.- + H+ Homo sapiens
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Homo sapiens P04179
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2.- + H+
-
 Homo sapiens O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+ the conserved, active-site residue Tyr34 mediates product inhibition Homo sapiens O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 tetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 manganese superoxide dismutase
-
 Homo sapiens
1.15.1.1 MnSOD
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
 assay at Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 6.4 10.9
-
 Homo sapiens