BRENDA - Enzyme Database

Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II)

Paterson, J.K.; Renkema, K.; Burden, L.; Halleck, M.S.; Schlegel, R.A.; Williamson, P.; Daleke, D.L.; Biochemistry 45, 5367-5376 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.1
additional information
the secretory granule Atp8a1 is activated by phospholipids binding to a specific site whose properties (PS selectivity, dependence upon glycerol but not serine, stereochemistry, and vanadate sensitivity) are similar to, but distinct from, the properties of the substrate binding site of the plasma membrane flippase, no activation by N-methylphosphatidylserine, phosphatidylserine-O-methyl ester, lysophosphatidylserine, glycerophosphoserine, and phosphoserine, Atp8a1 activities are insensitive to the stereochemistry of the serine headgroup of activating phospholipid
Mus musculus
7.6.2.1
phosphatidylethanolamine
slight activation
Mus musculus
7.6.2.1
phosphatidylglycerol
slight activation
Mus musculus
7.6.2.1
phosphatidylhomoserine
slight activation
Mus musculus
7.6.2.1
phosphatidylhydroxypropionate
slight activation
Mus musculus
7.6.2.1
phosphatidylserine
2.1fold activation, Atp8a1 is activated only by the naturally occurring sn-1,2-glycerol isomer of PS and not the sn-2,3-glycerol stereoisomer
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
7.6.2.1
expression of His-tagged Atp8a1 in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
Ca2+
-
Mus musculus
7.6.2.1
sulfhydryl reagents
-
Mus musculus
7.6.2.1
vanadate
-
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
plasma membrane
-
Mus musculus
5886
-
7.6.2.1
secretory granule
-
Mus musculus
30141
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.1
Mg2+
-
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.6.2.1
120000
-
x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylserine/in
Mus musculus
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles
ADP + phosphate + phosphatidylserine/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.1
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.6.2.1
recombinant His-tagged Atp8a1 from Spodoptera frugiperda Sf21 cells by microsome preparation and nickel affinity chromatography
Mus musculus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.1
2.3
-
Atp8a1 in Sf21 cell lysate, in absence of phosphatidylserine
Mus musculus
7.6.2.1
4.9
-
Atp8a1 in Sf21 cell lysate, in presence of phosphatidylserine
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylserine/in
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles
685075
Mus musculus
ADP + phosphate + phosphatidylserine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/in
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry
685075
Mus musculus
ADP + phosphate + phosphatidylserine/out
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.6.2.1
?
x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.1
additional information
the secretory granule Atp8a1 is activated by phospholipids binding to a specific site whose properties (PS selectivity, dependence upon glycerol but not serine, stereochemistry, and vanadate sensitivity) are similar to, but distinct from, the properties of the substrate binding site of the plasma membrane flippase, no activation by N-methylphosphatidylserine, phosphatidylserine-O-methyl ester, lysophosphatidylserine, glycerophosphoserine, and phosphoserine, Atp8a1 activities are insensitive to the stereochemistry of the serine headgroup of activating phospholipid
Mus musculus
7.6.2.1
phosphatidylethanolamine
slight activation
Mus musculus
7.6.2.1
phosphatidylglycerol
slight activation
Mus musculus
7.6.2.1
phosphatidylhomoserine
slight activation
Mus musculus
7.6.2.1
phosphatidylhydroxypropionate
slight activation
Mus musculus
7.6.2.1
phosphatidylserine
2.1fold activation, Atp8a1 is activated only by the naturally occurring sn-1,2-glycerol isomer of PS and not the sn-2,3-glycerol stereoisomer
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.1
expression of His-tagged Atp8a1 in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
Ca2+
-
Mus musculus
7.6.2.1
sulfhydryl reagents
-
Mus musculus
7.6.2.1
vanadate
-
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
plasma membrane
-
Mus musculus
5886
-
7.6.2.1
secretory granule
-
Mus musculus
30141
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.1
Mg2+
-
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.6.2.1
120000
-
x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylserine/in
Mus musculus
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles
ADP + phosphate + phosphatidylserine/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.1
recombinant His-tagged Atp8a1 from Spodoptera frugiperda Sf21 cells by microsome preparation and nickel affinity chromatography
Mus musculus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.1
2.3
-
Atp8a1 in Sf21 cell lysate, in absence of phosphatidylserine
Mus musculus
7.6.2.1
4.9
-
Atp8a1 in Sf21 cell lysate, in presence of phosphatidylserine
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylserine/in
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles
685075
Mus musculus
ADP + phosphate + phosphatidylserine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/in
selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry
685075
Mus musculus
ADP + phosphate + phosphatidylserine/out
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.6.2.1
?
x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE
Mus musculus