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Literature summary extracted from

  • Knol, J.; Bodewits, K.; Hessels, G.I.; Dijkhuizen, L.; van der Geize, R.
    3-Keto-5alpha-steroid DELTA(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism (2008), Biochem. J., 410, 339-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 expressed in Escherichia coli BL21(DE3) cells Rhodococcus erythropolis

General Stability

EC Number General Stability Organism
1.3.99.4 KSTD3 activity is lost upon purification using Ni-NTA column chromatography Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.4 0.003
-
 progesterone apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.005
-
 4-androstene-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.006
-
 5alpha-androstan-3-one-17beta-ol apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.007
-
 5alpha-pregnane-3,20-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.013
-
 5alpha-androstane-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.014
-
 5alpha-pregnane-3,20-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.018
-
 progesterone apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.021
-
 5alpha-pregnane-3,20-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.026
-
 23,24-bis-nor-5alpha-cholestan-3-one acid apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.029
-
 progesterone apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.033
-
 5alpha-androstane-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Mycobacterium tuberculosis
1.3.99.4 0.036
-
 5alpha-androstane-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.036
-
 5alpha-androstan-3-one-17beta-ol apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.058
-
 9alpha-hydroxy-4-androstene-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.059
-
 5alpha-androstane-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.064
-
 11beta-cortisol apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.065
-
 progesterone apparent value, in 50 mM Tris (pH 7.4), at 30°C Mycobacterium tuberculosis
1.3.99.4 0.07
-
 5alpha-androstan-3-one-17beta-ol apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.074
-
 23,24-bis-nor-5alpha-cholestan-3-one acid apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.104
-
 4-androstene-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.119
-
 9alpha-hydroxy-4-androstene-3,17-dione apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis
1.3.99.4 0.166
-
 5alpha-androstan-3-one-17beta-ol apparent value, in 50 mM Tris (pH 7.4), at 30°C Mycobacterium tuberculosis
1.3.99.4 0.557
-
 11beta-cortisol apparent value, in 50 mM Tris (pH 7.4), at 30°C Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.4 53000
-
 SDS-PAGE Rhodococcus erythropolis
1.3.99.4 60000
-
 SDS-PAGE Rhodococcus erythropolis
1.3.99.4 61000
-
 SDS-PAGE Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Mycobacterium tuberculosis
-
-
-
1.3.99.4 Mycobacterium tuberculosis H37Rv
-
-
-
1.3.99.4 Rhodococcus erythropolis A9QAE5 isozyme KSTD3
-
1.3.99.4 Rhodococcus erythropolis Q8RLT5 isozyme KSTD2
-
1.3.99.4 Rhodococcus erythropolis Q9RA02 isozyme KSTD1
-
1.3.99.4 Rhodococcus erythropolis SQ1 A9QAE5 isozyme KSTD3
-
1.3.99.4 Rhodococcus erythropolis SQ1 Q8RLT5 isozyme KSTD2
-
1.3.99.4 Rhodococcus erythropolis SQ1 Q9RA02 isozyme KSTD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.4 KSTD3 activity is lost upon purification using Ni-NTA column chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 11beta-cortisol + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis (11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 11beta-cortisol + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis SQ1 (11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis ?
-
 ?
1.3.99.4 23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis SQ1 ?
-
 ?
1.3.99.4 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis 1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis SQ1 1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 4-pregnene-3,20-dione + acceptor
-
 Rhodococcus erythropolis 1,4-pregnene-3,20 dione + reduced acceptor
-
 ?
1.3.99.4 4-pregnene-3,20-dione + acceptor
-
 Rhodococcus erythropolis SQ1 1,4-pregnene-3,20 dione + reduced acceptor
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one Rhodococcus erythropolis (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one Mycobacterium tuberculosis (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one Rhodococcus erythropolis SQ1 (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis SQ1 (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one Mycobacterium tuberculosis H37Rv (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity with 5alpha-androstane-3,17-dione Mycobacterium tuberculosis 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity with 5alpha-androstane-3,17-dione Rhodococcus erythropolis 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity with 5alpha-androstane-3,17-dione Rhodococcus erythropolis SQ1 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol KSTD3 shows highest activity with 5alpha-androstane-3,17-dione Mycobacterium tuberculosis H37Rv 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis 5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis SQ1 5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol preferred substrate of isozyme KSTD2 Rhodococcus erythropolis 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis 9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 additional information 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis ?
-
 ?
1.3.99.4 additional information 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates Mycobacterium tuberculosis ?
-
 ?
1.3.99.4 additional information 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis ?
-
 ?
1.3.99.4 additional information 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis ?
-
 ?
1.3.99.4 additional information 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis SQ1 ?
-
 ?
1.3.99.4 additional information 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis SQ1 ?
-
 ?
1.3.99.4 additional information 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates Rhodococcus erythropolis SQ1 ?
-
 ?
1.3.99.4 additional information 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates Mycobacterium tuberculosis H37Rv ?
-
 ?
1.3.99.4 progesterone + 2,6-dichlorophenol-indophenol
-
 Mycobacterium tuberculosis pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 progesterone + 2,6-dichlorophenol-indophenol
-
 Rhodococcus erythropolis pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 progesterone + 2,6-dichlorophenol-indophenol
-
 Mycobacterium tuberculosis H37Rv pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-keto-5alpha-steroid DELTA(1)-dehydrogenase
-
 Mycobacterium tuberculosis
1.3.99.4 3-keto-5alpha-steroid DELTA(1)-dehydrogenase
-
 Rhodococcus erythropolis
1.3.99.4 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
-
 Mycobacterium tuberculosis
1.3.99.4 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
-
 Rhodococcus erythropolis
1.3.99.4 KSTD1 isozyme Rhodococcus erythropolis
1.3.99.4 KSTD2 isozyme Rhodococcus erythropolis
1.3.99.4 KSTD3 isozyme Rhodococcus erythropolis
1.3.99.4 KSTD3 orthologue
-
 Mycobacterium tuberculosis
1.3.99.4 Rv3537
-
 Mycobacterium tuberculosis