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Literature summary extracted from

  • Logan, F.J.; Taylor, M.C.; Wilkinson, S.R.; Kaur, H.; Kelly, J.M.
    The terminal step in vitamin C biosynthesis in Trypanosoma cruzi is mediated by a FMN-dependent galactonolactone oxidase (2007), Biochem. J., 407, 419-426.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.12 expressed in Escherichia coli BL-21+ cells Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.12 H447G the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi
1.3.3.12 K450G the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity Trypanosoma cruzi
1.3.3.12 K55G the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity Trypanosoma cruzi
1.3.3.12 K55H the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%) Trypanosoma cruzi
1.3.3.12 K55L the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi
1.3.3.12 W448G the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.12 Hg2+ complete inhibition at 1 mM Trypanosoma cruzi
1.3.3.12 N-ethylmaleimide 77% inhibition at 1 mM Trypanosoma cruzi
1.3.3.12 p-chloromercuribenzoate complete inhibition at 1 mM Trypanosoma cruzi
1.3.3.12 Zn2+ 63% inhibition at 1 mM Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.12 10.82
-
 D-arabinono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi
1.3.3.12 11.22
-
 L-galactono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.12 57000
-
 SDS-PAGE Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.12 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.12 Ni-NTA column chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.12 amastigote
-
 Trypanosoma cruzi
-
1.3.3.12 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.12 D-arabinono-1,4-lactone + O2
-
 Trypanosoma cruzi ?
-
 ?
1.3.3.12 L-galactono-1,4-lactone + O2
-
 Trypanosoma cruzi L-ascorbate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.12 GAL
-
 Trypanosoma cruzi
1.3.3.12 galactonolactone oxidase
-
 Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.12 50
-
 heat-inactivated above 50°C Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.12 0.161
-
 L-galactono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi
1.3.3.12 0.285
-
 D-arabinono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.12 7.5 8
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.12 FMN dependent on non-covalently-bound FMN Trypanosoma cruzi