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Literature summary extracted from

  • Wiktelius, E.; Stenberg, G.
    Novel class of glutathione transferases from cyanobacteria exhibit high catalytic activities towards naturally occurring isothiocyanates (2007), Biochem. J., 406, 115-123.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Cibacron blue
-
 Synechococcus elongatus
2.5.1.18 Cibacron blue
-
 Thermosynechococcus vestitus
2.5.1.18 hexyl glutathione
-
 Synechococcus elongatus
2.5.1.18 hexyl glutathione
-
 Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.047
-
 glutathione at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
2.5.1.18 0.084
-
 glutathione at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus
2.5.1.18 0.16
-
 phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
2.5.1.18 0.75
-
 phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Synechococcus elongatus
-
 strain PCC 6301
-
2.5.1.18 Thermosynechococcus vestitus Q8DMB4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 glutathione Sepharose column chromatography Synechococcus elongatus
2.5.1.18 glutathione Sepharose column chromatography Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.038
-
 using H2O2 as a substrate Thermosynechococcus vestitus
2.5.1.18 0.04
-
 using 4-nitrobenzyl chloride as a substrate Thermosynechococcus vestitus
2.5.1.18 0.1
-
 using 4-nitrobenzylchloride as a substrate Synechococcus elongatus
2.5.1.18 0.14
-
 using 1-chloro-2,4-dinitrobenzene as a substrate Thermosynechococcus vestitus
2.5.1.18 0.15
-
 using ethacrynic acid as a substrate Thermosynechococcus vestitus
2.5.1.18 0.17
-
 using 1-chloro-2,4-dinitrobenzene as a substrate Synechococcus elongatus
2.5.1.18 0.24
-
 using cumene hydroperoxide as a substrate Thermosynechococcus vestitus
2.5.1.18 0.27
-
 using ethacrynic acid as a substrate Synechococcus elongatus
2.5.1.18 0.34
-
 using 1,2-epoxy-3-(p-nitrophenoxy)propane as a substrate Thermosynechococcus vestitus
2.5.1.18 0.42
-
 using 1,2-epoxy-3-(p-nitrophenoxy)propane as a substrate Synechococcus elongatus
2.5.1.18 0.75
-
 using cumene hydroperoxide as a substrate Synechococcus elongatus
2.5.1.18 1.54
-
 using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate Thermosynechococcus vestitus
2.5.1.18 5
-
 using sulforaphane as a substrate Thermosynechococcus vestitus
2.5.1.18 5.26
-
 using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate Synechococcus elongatus
2.5.1.18 6
-
 using propyl-isothiocyanate as a substrate Synechococcus elongatus
2.5.1.18 8
-
 using propyl-isothiocyanate as a substrate Thermosynechococcus vestitus
2.5.1.18 10.04
-
 using trans-2-nonenal as a substrate Thermosynechococcus vestitus
2.5.1.18 11
-
 using cyclohexyl-isothiocyanate as a substrate Thermosynechococcus vestitus
2.5.1.18 14
-
 using allyl-isothiocyanate as a substrate Thermosynechococcus vestitus
2.5.1.18 15
-
 using allyl-isothiocyanate as a substrate Synechococcus elongatus
2.5.1.18 21
-
 using cyclohexyl-isothiocyanate as a substrate Synechococcus elongatus
2.5.1.18 26
-
 using sulforaphane as a substrate Synechococcus elongatus
2.5.1.18 33
-
 using phenethyl-isothiocyanate as a substrate Thermosynechococcus vestitus
2.5.1.18 102
-
 using phenethyl-isothiocyanate as a substrate Synechococcus elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-epoxy-3-(p-nitrophenoxy)propane
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + 1,2-epoxy-3-(p-nitrophenoxy)propane
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Synechococcus elongatus S-(2,4-dinitrophenyl)-glutathione + HCl
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Thermosynechococcus vestitus S-(2,4-dinitrophenyl)-glutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + 4-nitrobenzylchloride
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + allyl-isothiocyanate
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + allyl-isothiocyanate
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + cyclohexyl-isothiocyanate
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + cyclohexyl-isothiocyanate
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + H2O2
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + phenethyl-isothiocyanate
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + phenethyl-isothiocyanate
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + propyl-isothiocyanate
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + propyl-isothiocyanate
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + sulforaphane
-
 Synechococcus elongatus ?
-
 ?
2.5.1.18 glutathione + sulforaphane
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 glutathione + trans-2-nonenal
-
 Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 additional information no activity with 1,2-dichloro-4-nitrobenzene, iodomethane, 1-iodopenthane, and dichloromethane Thermosynechococcus vestitus ?
-
 ?
2.5.1.18 additional information no activity with 1,2-dichloro-4-nitrobenzene, iodomethane, 1-iodopenthane, H2O2, trans-2-nonenal, and dichloromethane Synechococcus elongatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 Chi class GST
-
 Synechococcus elongatus
2.5.1.18 Chi class GST
-
 Thermosynechococcus vestitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 100
-
 almost 100% of its activity retained after incubation at 70°C Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 17
-
 glutathione at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
2.5.1.18 21.4
-
 phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
2.5.1.18 48.7
-
 glutathione at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus
2.5.1.18 63.4
-
 phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00000075
-
 at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus Cibacron blue
2.5.1.18 0.00005
-
 at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus Cibacron blue
2.5.1.18 0.15
-
 at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus hexyl glutathione
2.5.1.18 0.5
-
 at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus hexyl glutathione