EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.9 | expressed in Escherichia coli JM 109 | Candida sp. HA 167 |
1.1.1.14 | expression in Escherichia coli | Candida sp. HA 167 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.9 | E154C | mutant bearing a disrupted Zn2+ binding site: purified preparations show a variable Zn2+ (0.10-0.40 atom/subunit), mutant exhibits a constant catalytic Zn2+ centre activity and does not require exogenous Zn2+ for activity or stability. E154C retains 0.019% and 0.74% of wild-type catalytic efficiency (kcat/Km (sorbitol): 7800/Msec and kcat:161/sec) for NAD+-dependent oxidation of sorbitol at 25°C respectively. The pH profile of kcat/Ksorbitol for E154C decreases below an apparent pK of 9.1, reflecting a shift in pK by about +1.7-1.9 pH units compared with the corresponding pH profiles for wild-type. IC50 (ZnSO4): 0.005 mM | Candida sp. HA 167 |
1.1.1.14 | E154C | purified preparations of mutant contain 0.1-0.4 atoms of Zn2+ per subunit and exhibit a constant catalytic Zn2+ centre activity of 1.19 per s, mutant does not require exogenous Zn2+ for stability. Mutant retains less than 1% of wild-type catalytic efficiency and displays similar primary and solvent deuterium effects as wild-type | Candida sp. HA 167 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | Zn2+ | exogenous Zn2+ (added as ZnSO4) in the concentration range 1-100 microM is a strong irreversible inhibitor of wild-type and mutant E154C | Candida sp. HA 167 | |
1.1.1.9 | ZnSO4 | - |
Candida sp. HA 167 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 3 | - |
D-sorbitol | kcat/Km (sorbitol): 9500/Msec | Ovis aries | |
1.1.1.9 | 21 | - |
sorbitol | kcat/Km (sorbitol): 7800/Msec | Candida sp. HA 167 | |
1.1.1.9 | 290 | - |
NAD+ | mutant E154C, kcat/Km (NAD+): 4100/Msec | Candida sp. HA 167 | |
1.1.1.9 | 430 | - |
NAD+ | kcat/Km (NAD+): 370000/Msec | Candida sp. HA 167 | |
1.1.1.9 | 500 | - |
NAD+ | kcat/Km (NAD+): 57000/Msec | Ovis aries | |
1.1.1.9 | 785 | - |
sorbitol | mutant E154C, kcat/Km (sorbitol): 1.5/Msec | Candida sp. HA 167 | |
1.1.1.14 | 0.29 | - |
NAD+ | mutant E154C, pH 9.0, 25°C | Candida sp. HA 167 | |
1.1.1.14 | 0.43 | - |
NAD+ | wild-type, pH 9.0, 25°C | Candida sp. HA 167 | |
1.1.1.14 | 0.5 | - |
NAD+ | pH 9.0, 25°C | Ovis aries | |
1.1.1.14 | 3 | - |
D-sorbitol | pH 9.0, 25°C | Ovis aries | |
1.1.1.14 | 21 | - |
D-sorbitol | wild-type, pH 9.0, 25°C | Candida sp. HA 167 | |
1.1.1.14 | 785 | - |
D-sorbitol | mutant E154C, pH 9.0, 25°C | Candida sp. HA 167 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | Zn2+ | - |
Ovis aries | |
1.1.1.14 | Zn2+ | mutant E154C conatins 0.1-0.4 atoms per subunit | Candida sp. HA 167 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 160000 | - |
gel filtration, protein is a homotetramer | Candida sp. HA 167 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Candida sp. HA 167 | - |
- |
- |
1.1.1.9 | Ovis aries | - |
- |
- |
1.1.1.14 | Candida sp. HA 167 | - |
- |
- |
1.1.1.14 | Ovis aries | - |
commercial preparation | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | using affinity chromatography and preparative gel filtration | Candida sp. HA 167 |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.9 | liver | - |
Ovis aries | - |
1.1.1.14 | liver | - |
Ovis aries | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-sorbitol + NAD+ | - |
Ovis aries | D-fructose + NADH + H+ | - |
r | |
1.1.1.9 | sorbitol + NAD+ | - |
Candida sp. HA 167 | D-fructose + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NAD+ | - |
Ovis aries | D-xylulose + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NAD+ | - |
Candida sp. HA 167 | D-xylulose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | - |
Ovis aries | D-fructose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | - |
Candida sp. HA 167 | D-fructose + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.9 | homotetramer | gel filtration | Candida sp. HA 167 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.9 | GmXDH | - |
Candida sp. HA 167 |
1.1.1.9 | slSDH | - |
Ovis aries |
1.1.1.9 | xylitol dehydrogenase | - |
Ovis aries |
1.1.1.9 | xylitol dehydrogenase | - |
Candida sp. HA 167 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 25 | - |
assay at | Ovis aries |
1.1.1.9 | 25 | - |
assay at | Candida sp. HA 167 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 1.19 | - |
sorbitol | mutant E154C | Candida sp. HA 167 | |
1.1.1.9 | 2 | 8 | D-sorbitol | - |
Ovis aries | |
1.1.1.9 | 161 | - |
sorbitol | - |
Candida sp. HA 167 | |
1.1.1.14 | 1.19 | - |
D-sorbitol | mutant E154C, pH 9.0, 25°C | Candida sp. HA 167 | |
1.1.1.14 | 2 | 8 | D-sorbitol | pH 9.0, 25°C | Ovis aries | |
1.1.1.14 | 161 | - |
D-sorbitol | wild-type, pH 9.0, 25°C | Candida sp. HA 167 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 9 | - |
assay at | Ovis aries |
1.1.1.9 | 9 | - |
assay at | Candida sp. HA 167 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | NAD+ | - |
Ovis aries | |
1.1.1.14 | NAD+ | - |
Candida sp. HA 167 | |
1.1.1.14 | NADH | - |
Ovis aries | |
1.1.1.14 | NADH | - |
Candida sp. HA 167 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 0.005 | - |
value identical for wildtype and for mutant E154C | Candida sp. HA 167 | ZnSO4 |