Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Klimacek, M.; Hellmer, H.; Nidetzky, B.
    Catalytic mechanism of Zn2+-dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu154-Cys forms of yeast xylitol dehydrogenase (2007), Biochem. J., 404, 421-429.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 expressed in Escherichia coli JM 109 Candida sp. HA 167
1.1.1.14 expression in Escherichia coli Candida sp. HA 167

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 E154C mutant bearing a disrupted Zn2+ binding site: purified preparations show a variable Zn2+ (0.10-0.40 atom/subunit), mutant exhibits a constant catalytic Zn2+ centre activity and does not require exogenous Zn2+ for activity or stability. E154C retains 0.019% and 0.74% of wild-type catalytic efficiency (kcat/Km (sorbitol): 7800/Msec and kcat:161/sec) for NAD+-dependent oxidation of sorbitol at 25°C respectively. The pH profile of kcat/Ksorbitol for E154C decreases below an apparent pK of 9.1, reflecting a shift in pK by about +1.7-1.9 pH units compared with the corresponding pH profiles for wild-type. IC50 (ZnSO4): 0.005 mM Candida sp. HA 167
1.1.1.14 E154C purified preparations of mutant contain 0.1-0.4 atoms of Zn2+ per subunit and exhibit a constant catalytic Zn2+ centre activity of 1.19 per s, mutant does not require exogenous Zn2+ for stability. Mutant retains less than 1% of wild-type catalytic efficiency and displays similar primary and solvent deuterium effects as wild-type Candida sp. HA 167

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.9 Zn2+ exogenous Zn2+ (added as ZnSO4) in the concentration range 1-100 microM is a strong irreversible inhibitor of wild-type and mutant E154C Candida sp. HA 167
1.1.1.9 ZnSO4
-
Candida sp. HA 167

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.9 3
-
D-sorbitol kcat/Km (sorbitol): 9500/Msec Ovis aries
1.1.1.9 21
-
sorbitol kcat/Km (sorbitol): 7800/Msec Candida sp. HA 167
1.1.1.9 290
-
NAD+ mutant E154C, kcat/Km (NAD+): 4100/Msec Candida sp. HA 167
1.1.1.9 430
-
NAD+ kcat/Km (NAD+): 370000/Msec Candida sp. HA 167
1.1.1.9 500
-
NAD+ kcat/Km (NAD+): 57000/Msec Ovis aries
1.1.1.9 785
-
sorbitol mutant E154C, kcat/Km (sorbitol): 1.5/Msec Candida sp. HA 167
1.1.1.14 0.29
-
NAD+ mutant E154C, pH 9.0, 25°C Candida sp. HA 167
1.1.1.14 0.43
-
NAD+ wild-type, pH 9.0, 25°C Candida sp. HA 167
1.1.1.14 0.5
-
NAD+ pH 9.0, 25°C Ovis aries
1.1.1.14 3
-
D-sorbitol pH 9.0, 25°C Ovis aries
1.1.1.14 21
-
D-sorbitol wild-type, pH 9.0, 25°C Candida sp. HA 167
1.1.1.14 785
-
D-sorbitol mutant E154C, pH 9.0, 25°C Candida sp. HA 167

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.14 Zn2+
-
Ovis aries
1.1.1.14 Zn2+ mutant E154C conatins 0.1-0.4 atoms per subunit Candida sp. HA 167

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.9 160000
-
gel filtration, protein is a homotetramer Candida sp. HA 167

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Candida sp. HA 167
-
-
-
1.1.1.9 Ovis aries
-
-
-
1.1.1.14 Candida sp. HA 167
-
-
-
1.1.1.14 Ovis aries
-
commercial preparation
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 using affinity chromatography and preparative gel filtration Candida sp. HA 167

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.9 liver
-
Ovis aries
-
1.1.1.14 liver
-
Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-sorbitol + NAD+
-
Ovis aries D-fructose + NADH + H+
-
r
1.1.1.9 sorbitol + NAD+
-
Candida sp. HA 167 D-fructose + NADH + H+
-
?
1.1.1.9 xylitol + NAD+
-
Ovis aries D-xylulose + NADH + H+
-
?
1.1.1.9 xylitol + NAD+
-
Candida sp. HA 167 D-xylulose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+
-
Ovis aries D-fructose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+
-
Candida sp. HA 167 D-fructose + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.9 homotetramer gel filtration Candida sp. HA 167

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 GmXDH
-
Candida sp. HA 167
1.1.1.9 slSDH
-
Ovis aries
1.1.1.9 xylitol dehydrogenase
-
Ovis aries
1.1.1.9 xylitol dehydrogenase
-
Candida sp. HA 167

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 25
-
assay at Ovis aries
1.1.1.9 25
-
assay at Candida sp. HA 167

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.9 1.19
-
sorbitol mutant E154C Candida sp. HA 167
1.1.1.9 2 8 D-sorbitol
-
Ovis aries
1.1.1.9 161
-
sorbitol
-
Candida sp. HA 167
1.1.1.14 1.19
-
D-sorbitol mutant E154C, pH 9.0, 25°C Candida sp. HA 167
1.1.1.14 2 8 D-sorbitol pH 9.0, 25°C Ovis aries
1.1.1.14 161
-
D-sorbitol wild-type, pH 9.0, 25°C Candida sp. HA 167

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 9
-
assay at Ovis aries
1.1.1.9 9
-
assay at Candida sp. HA 167

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
Ovis aries
1.1.1.14 NAD+
-
Candida sp. HA 167
1.1.1.14 NADH
-
Ovis aries
1.1.1.14 NADH
-
Candida sp. HA 167

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.9 0.005
-
value identical for wildtype and for mutant E154C Candida sp. HA 167 ZnSO4