EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.1 | R206H | Arg206 to histidine followed by covalent coupling of mPEG-SNHS [methoxypoly(ethyene glycol) succinate N-hydroxysuccinimide ester] to the mutant enzyme results in an improved modified form of EcaL-ASNase that retains 82% of the original catalytic activity, exhibits enhanced resistance to trypsin degradation, and has higher thermal stability compared with the wild-type enzyme | Pectobacterium carotovorum |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.1 | limited proteolysis of EcaL-ASNase with trypsin is associated with a first cleavage of the peptide bond between Lys53 and Gly54, and then a second cleavage at Arg206-Ser207 of the C-terminal fragment, peptide 54-327. Site-directed mutagenesis of Arg206 to histidine followed by covalent coupling of mPEG-SNHS [methoxypoly(ethylene glycol) succinate N-hydroxysuccinimide ester] to the mutant enzyme results in an improved modified form of EcaL-ASNase that retains 82% of the original catalytic activity, exhibits enhanced resistance to trypsin degradation, and has higher thermal stability compared with the wild-type enzyme | Pectobacterium carotovorum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.36 | - |
L-asparagine | 37°C, mutant enzyme R206H covalently coupled to methoxypoly(ethyene glycol) succinate N-hydroxysuccinimide ester | Pectobacterium carotovorum | |
3.5.1.1 | 0.55 | - |
L-asparagine | 37°C, mutant enzyme R206H | Pectobacterium carotovorum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Pectobacterium carotovorum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.1 | EcaL-ASNase | - |
Pectobacterium carotovorum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 39 | - |
Tm-value of wild-type enzyme is 38.9°C, Tm-value of mutant enzyme is 38.8°C | Pectobacterium carotovorum |
3.5.1.1 | 43 | - |
Tm-value of wild-type enzyme covalently coupled to methoxypoly(ethyene glycol) succinate N-hydroxysuccinimide ester is 42.9°C, Tm-value of mutant enzyme R206H covalently coupled to methoxypoly(ethyene glycol) succinate N-hydroxysuccinimide ester is 43.1°C | Pectobacterium carotovorum |