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Literature summary extracted from

  • Ding, D.; Sagher, D.; Laugier, E.; Rey, P.; Weissbach, H.; Zhang, X.H.
    Studies on the reducing systems for plant and animal thioredoxin-independent methionine sulfoxide reductases B (2007), Biochem. Biophys. Res. Commun., 361, 629-633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.B3 expression in Escherichia coli BL21 Nicotiana tabacum
1.8.4.12 expression in Escherichia coli BL21 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.B3 chloroplast
-
 Nicotiana tabacum 9507
-
1.8.4.12 chloroplast
-
 Nicotiana tabacum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.B3 15200
-
 x * 15200, calculated from sequence Nicotiana tabacum
1.8.4.12 14800
-
 x * 14800, calculated from sequence Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.B3 peptide-L-methionine-(R)-S-sulfoxide + thionein Nicotiana tabacum
-
 peptide-L-methionine + thionein disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.B3 Nicotiana tabacum A7U630 precursor, fragment
-
1.8.4.12 Nicotiana tabacum A7U629 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.B3
-
 Nicotiana tabacum
1.8.4.12
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.B3 leaf
-
 Nicotiana tabacum
-
1.8.4.12 leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + bovine liver thionein + H2O
-
 ?
1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32 NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + ?
-
 ?
1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
-
 ?
1.8.4.B3 additional information NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction Nicotiana tabacum ?
-
 ?
1.8.4.B3 peptide-L-methionine-(R)-S-sulfoxide + thionein
-
 Nicotiana tabacum peptide-L-methionine + thionein disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + thioredoxin activity with Escherichia coli thioredoxin, NtMsrB21 Nicotiana tabacum dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 additional information NtMsrB1 shows no activity with dithiothreitol Nicotiana tabacum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.B3 ? x * 15200, calculated from sequence Nicotiana tabacum
1.8.4.12 ? x * 14800, calculated from sequence Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
1.8.4.B3 NtMsrB1
-
 Nicotiana tabacum
1.8.4.B3 thioredoxin-independent methionine sulfoxide reductases B
-
 Nicotiana tabacum
1.8.4.12 NtMsrB2
-
 Nicotiana tabacum