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Literature summary extracted from

  • Assimes, T.L.; Knowles, J.W.; Priest, J.R.; Basu, A.; Borchert, A.; Volcik, K.A.; Grove, M.L.; Tabor, H.K.; Southwick, A.; Tabibiazar, R.; Sidney, S.; Boerwinkle, E.; Go, A.S.; Iribarren, C.; Hlatky, M.A.; Fortmann, S.P.; Myers, R.M.; Kuhn, H.; Risch, N.; Quertermous, T.
    A near null variant of 12/15-LOX encoded by a novel SNP in ALOX15 and the risk of coronary artery disease (2008), Atherosclerosis, 198, 136-144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme Homo sapiens
1.13.11.33 gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 additional information 12/15-LO knockout mice show reduced monocyte chemoattractant protein MCP-1 expression Mus musculus
1.13.11.31 T560M naturally occurring enzyme mutant, the mutant shows 20fold reduced catalytic activity, genotyping using 12974 samples, the haplotypes show increased risk of coronary artery disease compared to non-carriers, overview Homo sapiens
1.13.11.33 T560M naturally occurring enzyme mutant, the mutant shows 20fold reduced catalytic activity, genotyping using 12974 samples, the haplotypes show increased risk of coronary artery disease compared to non-carriers, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.31 2 arachidonate + 2 O2 + 2 H+ Mus musculus
-
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
1.13.11.31 2 arachidonate + 2 O2 + 2 H+ Mus musculus C57BL/6
-
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
gene ALOX15
-
1.13.11.31 Mus musculus
-
C57BL/6 mice
-
1.13.11.31 Mus musculus C57BL/6
-
C57BL/6 mice
-
1.13.11.33 Homo sapiens P16050 gene ALOX15
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 J-774A.1 cell macrophage cell line Mus musculus
-
1.13.11.31 macrophage peritoneal Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 2 arachidonate + 2 O2 + 2 H+
-
Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
1.13.11.31 2 arachidonate + 2 O2 + 2 H+
-
Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product ?
1.13.11.31 2 arachidonate + 2 O2 + 2 H+
-
Mus musculus C57BL/6 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview ?
1.13.11.31 2 arachidonate + 2 O2 + 2 H+
-
Mus musculus C57BL/6 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product ?
1.13.11.31 additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity Homo sapiens ?
-
?
1.13.11.31 additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview Mus musculus ?
-
?
1.13.11.31 additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview Mus musculus C57BL/6 ?
-
?
1.13.11.33 additional information a bifunctional enzyme exhibiting 12-LO and 15-LO activity Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-lipoxygenase
-
Mus musculus
1.13.11.31 12/15-lipoxygenase
-
Mus musculus
1.13.11.31 12/15-lipoxygenase
-
Homo sapiens
1.13.11.31 12/15-LO
-
Mus musculus
1.13.11.31 12/15-LO
-
Homo sapiens
1.13.11.33 12/15-lipoxygenase
-
Homo sapiens
1.13.11.33 12/15-LO
-
Homo sapiens