EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.31 | gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme | Homo sapiens |
1.13.11.33 | gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.31 | additional information | 12/15-LO knockout mice show reduced monocyte chemoattractant protein MCP-1 expression | Mus musculus |
1.13.11.31 | T560M | naturally occurring enzyme mutant, the mutant shows 20fold reduced catalytic activity, genotyping using 12974 samples, the haplotypes show increased risk of coronary artery disease compared to non-carriers, overview | Homo sapiens |
1.13.11.33 | T560M | naturally occurring enzyme mutant, the mutant shows 20fold reduced catalytic activity, genotyping using 12974 samples, the haplotypes show increased risk of coronary artery disease compared to non-carriers, overview | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | Mus musculus | - |
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview | ? | |
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | Mus musculus C57BL/6 | - |
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.31 | Homo sapiens | - |
gene ALOX15 | - |
1.13.11.31 | Mus musculus | - |
C57BL/6 mice | - |
1.13.11.31 | Mus musculus C57BL/6 | - |
C57BL/6 mice | - |
1.13.11.33 | Homo sapiens | P16050 | gene ALOX15 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.31 | J-774A.1 cell | macrophage cell line | Mus musculus | - |
1.13.11.31 | macrophage | peritoneal | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | - |
Mus musculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview | ? | |
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | - |
Mus musculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product | ? | |
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | - |
Mus musculus C57BL/6 | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate increases expression of monocyte chemoattractant protein MCP-1 in macrophages, also but less by 15(S)-hydroxyeicosatetranoic acid, and 12(S)-HETE activates NADPH oxidase, overview | ? | |
1.13.11.31 | 2 arachidonate + 2 O2 + 2 H+ | - |
Mus musculus C57BL/6 | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate + (5Z,8Z,10E,14Z)-(12S)-12-hydroxyeicosa-5,8,10,14-tetraenoate + H2O | i.e. 12(S)-HPETE and 12(S)-HETE, the first is the predominant product | ? | |
1.13.11.31 | additional information | a bifunctional enzyme exhibiting 12-LO and 15-LO activity | Homo sapiens | ? | - |
? | |
1.13.11.31 | additional information | a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview | Mus musculus | ? | - |
? | |
1.13.11.31 | additional information | a bifunctional enzyme exhibiting 12-LO and 15-LO activity, the enzyme is also able to catalyze stereoselective oxidation of linoleic acid at position 13 over 9 to preferentially form 13(S)-hydroperoxyoctadienoic acid, which enhances MCP-1 expression, overview | Mus musculus C57BL/6 | ? | - |
? | |
1.13.11.33 | additional information | a bifunctional enzyme exhibiting 12-LO and 15-LO activity | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.31 | 12-lipoxygenase | - |
Mus musculus |
1.13.11.31 | 12/15-lipoxygenase | - |
Mus musculus |
1.13.11.31 | 12/15-lipoxygenase | - |
Homo sapiens |
1.13.11.31 | 12/15-LO | - |
Mus musculus |
1.13.11.31 | 12/15-LO | - |
Homo sapiens |
1.13.11.33 | 12/15-lipoxygenase | - |
Homo sapiens |
1.13.11.33 | 12/15-LO | - |
Homo sapiens |