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Literature summary extracted from

  • Krejcik, Z.; Denger, K.; Weinitschke, S.; Hollemeyer, K.; Paces, V.; Cook, A.M.; Smits, T.H.
    Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis: purification of sulfoacetaldehyde dehydrogenase (2008), Arch. Microbiol., 190, 159-168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.73 gene safD, DNA and amino acid sequence determination and analysis, genetic organization Neptuniibacter caesariensis

General Stability

EC Number General Stability Organism
1.2.1.73 SafD is stable in Tris-HCl buffer, pH 9.0 Neptuniibacter caesariensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.73 NADP+ low activity an inhibition at higher concentration Neptuniibacter caesariensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.73 0.12
-
 NAD+ pH 9.0, 22°C Neptuniibacter caesariensis
1.2.1.73 0.49
-
 Sulfoacetaldehyde pH 9.0, 22°C Neptuniibacter caesariensis
1.2.1.73 4.5
-
 NADP+ pH 9.0, 22°C Neptuniibacter caesariensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.73 cytoplasm
-
 Neptuniibacter caesariensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.73 Mg2+
-
 Neptuniibacter caesariensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.73 49100
-
 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis
1.2.1.73 50000
-
 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis
1.2.1.73 190000
-
 gel filtration Neptuniibacter caesariensis
1.2.1.73 196500
-
 sequence calculation Neptuniibacter caesariensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.73 2-sulfoacetaldehyde + H2O + NAD+ Neptuniibacter caesariensis
-
 sulfoacetate + NADH + H+
-
 ?
1.2.1.73 additional information Neptuniibacter caesariensis the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.73 Neptuniibacter caesariensis
-
 formerly Oceanospirillum sp., CCUG 52065, a marine gammaproteobacterium, strain MED92, gene safD encoding an inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.73 native enzyme 610fold to homogeneity by anion exchange and hydrophobic interaction chromatography Neptuniibacter caesariensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.73 2-sulfoacetaldehyde + H2O + NAD(P)+ the enzyme is specific for sulfoacetaldehyde, NAD+ is the highly preferred cofactor to NADP+ Neptuniibacter caesariensis sulfoacetate + NAD(P)H + H+
-
 ?
1.2.1.73 2-sulfoacetaldehyde + H2O + NAD+
-
 Neptuniibacter caesariensis sulfoacetate + NADH + H+
-
 ?
1.2.1.73 additional information the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview Neptuniibacter caesariensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.73 tetramer 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.73 More the enzyme belongs to the NAD-dependent aldehyde dehydrogenase superfamily Neptuniibacter caesariensis
1.2.1.73 SafD
-
 Neptuniibacter caesariensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.73 22
-
 assay at room temperature Neptuniibacter caesariensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.73 9
-
-
 Neptuniibacter caesariensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.73 7.2 10.5 50% of maximal activity at pH 7.2, 70% at pH 8.0, and 80% at pH 9.5-10.5 Neptuniibacter caesariensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.73 NAD+ highly preferred cofactor Neptuniibacter caesariensis
1.2.1.73 NADP+ low activity and inhibition at higher concentration Neptuniibacter caesariensis