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Literature summary extracted from

  • Hilbi, H.; Dimroth, P.
    Purification and characterization of a cytoplasmic enzyme component of the Na+-activated malonate decarboxylase system of Malonomonas rubra: acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase (1994), Arch. Microbiol., 162, 48-56.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.10 citrate
-
 Malonomonas rubra
2.1.3.10 malonate
-
 Malonomonas rubra

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.10 0.5
-
 malonate pH 6.0 Malonomonas rubra
2.1.3.10 1.9
-
 malonyl-CoA pH 6.0 Malonomonas rubra
2.1.3.10 6.9
-
 acetyl-CoA pH 6.0 Malonomonas rubra
2.1.3.10 54
-
 acetate pH 6.0 Malonomonas rubra

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.10 59000
-
 gel filtration Malonomonas rubra
2.1.3.10 67000
-
 1 * 67000, SDS-PAGE Malonomonas rubra
7.2.4.4 67000
-
 x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase Malonomonas rubra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.10 acetyl-CoA + dephospho-CoA Malonomonas rubra
-
 CoA + acetyl-dephospho-CoA
-
 r
2.1.3.10 malonyl-CoA + dephospho-CoA Malonomonas rubra
-
 CoA + malonyl-dephospho-CoA
-
 r
2.1.3.10 additional information Malonomonas rubra enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues ?
-
 ?
7.2.4.4 additional information Malonomonas rubra subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.10 Malonomonas rubra
-
 enzyme is part of the Na+-activied malonate decarboxylase system
-
7.2.4.4 Malonomonas rubra
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.10 purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase Malonomonas rubra
7.2.4.4 purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex Malonomonas rubra

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.10 367
-
-
 Malonomonas rubra
7.2.4.4 42
-
 pH 6.0 Malonomonas rubra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.10 acetyl-CoA + dephospho-CoA
-
 Malonomonas rubra CoA + acetyl-dephospho-CoA
-
 r
2.1.3.10 acetyl-CoA + malonate
-
 Malonomonas rubra malonyl-CoA + acetate
-
 r
2.1.3.10 malonyl-CoA + acetate
-
 Malonomonas rubra acetyl-CoA + malonate
-
 r
2.1.3.10 malonyl-CoA + dephospho-CoA
-
 Malonomonas rubra CoA + malonyl-dephospho-CoA
-
 r
2.1.3.10 additional information enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues Malonomonas rubra ?
-
 ?
7.2.4.4 malonate + H+
-
 Malonomonas rubra acetate + CO2
-
 ?
7.2.4.4 additional information subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues Malonomonas rubra ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.10 monomer 1 * 67000, SDS-PAGE Malonomonas rubra
7.2.4.4 ? x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase Malonomonas rubra

Synonyms

EC Number Synonyms Comment Organism
2.1.3.10 acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase
-
 Malonomonas rubra
7.2.4.4 Na+-activated malonate decarboxylase
-
 Malonomonas rubra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.10 5.5
-
-
 Malonomonas rubra

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.10 4.5
-
 sharp decrease in activity from pH 5.5 to pH 4.5 Malonomonas rubra

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.10 0.4
-
 malonate pH 6.0 Malonomonas rubra
2.1.3.10 3
-
 citrate pH 6.0 Malonomonas rubra