BRENDA - Enzyme Database

Purification and characterization of a cytoplasmic enzyme component of the Na+-activated malonate decarboxylase system of Malonomonas rubra: acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase

Hilbi, H.; Dimroth, P.; Arch. Microbiol. 162, 48-56 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.3.10
citrate
-
Malonomonas rubra
2.1.3.10
malonate
-
Malonomonas rubra
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.3.10
0.5
-
malonate
pH 6.0
Malonomonas rubra
2.1.3.10
1.9
-
malonyl-CoA
pH 6.0
Malonomonas rubra
2.1.3.10
6.9
-
acetyl-CoA
pH 6.0
Malonomonas rubra
2.1.3.10
54
-
acetate
pH 6.0
Malonomonas rubra
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.10
59000
-
gel filtration
Malonomonas rubra
2.1.3.10
67000
-
1 * 67000, SDS-PAGE
Malonomonas rubra
7.2.4.4
67000
-
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.3.10
acetyl-CoA + dephospho-CoA
Malonomonas rubra
-
CoA + acetyl-dephospho-CoA
-
-
r
2.1.3.10
malonyl-CoA + dephospho-CoA
Malonomonas rubra
-
CoA + malonyl-dephospho-CoA
-
-
r
2.1.3.10
additional information
Malonomonas rubra
enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
7.2.4.4
additional information
Malonomonas rubra
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.3.10
Malonomonas rubra
-
enzyme is part of the Na+-activied malonate decarboxylase system
-
7.2.4.4
Malonomonas rubra
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.3.10
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
7.2.4.4
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex
Malonomonas rubra
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.3.10
367
-
-
Malonomonas rubra
7.2.4.4
42
-
pH 6.0
Malonomonas rubra
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.10
acetyl-CoA + dephospho-CoA
-
684745
Malonomonas rubra
CoA + acetyl-dephospho-CoA
-
-
-
r
2.1.3.10
malonyl-CoA + dephospho-CoA
-
684745
Malonomonas rubra
CoA + malonyl-dephospho-CoA
-
-
-
r
2.1.3.10
additional information
enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
7.2.4.4
malonate + H+
-
684745
Malonomonas rubra
acetate + CO2
-
-
-
?
7.2.4.4
additional information
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.3.10
monomer
1 * 67000, SDS-PAGE
Malonomonas rubra
7.2.4.4
?
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.10
5.5
-
-
Malonomonas rubra
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.3.10
4.5
-
sharp decrease in activity from pH 5.5 to pH 4.5
Malonomonas rubra
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.3.10
0.4
-
malonate
pH 6.0
Malonomonas rubra
2.1.3.10
3
-
citrate
pH 6.0
Malonomonas rubra
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.3.10
citrate
-
Malonomonas rubra
2.1.3.10
malonate
-
Malonomonas rubra
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.3.10
0.4
-
malonate
pH 6.0
Malonomonas rubra
2.1.3.10
3
-
citrate
pH 6.0
Malonomonas rubra
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.3.10
0.5
-
malonate
pH 6.0
Malonomonas rubra
2.1.3.10
1.9
-
malonyl-CoA
pH 6.0
Malonomonas rubra
2.1.3.10
6.9
-
acetyl-CoA
pH 6.0
Malonomonas rubra
2.1.3.10
54
-
acetate
pH 6.0
Malonomonas rubra
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.10
59000
-
gel filtration
Malonomonas rubra
2.1.3.10
67000
-
1 * 67000, SDS-PAGE
Malonomonas rubra
7.2.4.4
67000
-
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.3.10
acetyl-CoA + dephospho-CoA
Malonomonas rubra
-
CoA + acetyl-dephospho-CoA
-
-
r
2.1.3.10
malonyl-CoA + dephospho-CoA
Malonomonas rubra
-
CoA + malonyl-dephospho-CoA
-
-
r
2.1.3.10
additional information
Malonomonas rubra
enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
7.2.4.4
additional information
Malonomonas rubra
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.3.10
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
7.2.4.4
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex
Malonomonas rubra
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.3.10
367
-
-
Malonomonas rubra
7.2.4.4
42
-
pH 6.0
Malonomonas rubra
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.10
acetyl-CoA + dephospho-CoA
-
684745
Malonomonas rubra
CoA + acetyl-dephospho-CoA
-
-
-
r
2.1.3.10
malonyl-CoA + dephospho-CoA
-
684745
Malonomonas rubra
CoA + malonyl-dephospho-CoA
-
-
-
r
2.1.3.10
additional information
enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
7.2.4.4
malonate + H+
-
684745
Malonomonas rubra
acetate + CO2
-
-
-
?
7.2.4.4
additional information
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.3.10
monomer
1 * 67000, SDS-PAGE
Malonomonas rubra
7.2.4.4
?
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.10
5.5
-
-
Malonomonas rubra
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.3.10
4.5
-
sharp decrease in activity from pH 5.5 to pH 4.5
Malonomonas rubra