Literature summary extracted from
Smeets, A.; Marchand, C.; Linard, D.; Knoops, B.; Declercq, J.P.
The crystal structures of oxidized forms of human peroxiredoxin 5 with an intramolecular disulfide bond confirm the proposed enzymatic mechanism for atypical 2-Cys peroxiredoxins (2008), Arch. Biochem. Biophys., 477, 98-104.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.11.1.24 |
the C72S mutant is crystallized by hanging drop vapour diffusion method with sodium cacodylate 0.1 M pH 6.5, PEG8000 20% (w/v), sodium acetate 0.1 M, and 6-aminocaproic acid 3% (w/v), at 18°C |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.11.1.24 |
C72S |
the C72S mutation improves the crystallization in oxidizing conditions |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.11.1.24 |
benzoate |
in orthorhombic 1 form, the benzoate ion is present close to the Cp residue in both fully folded subunits the benzoate ion is not observed in the six oxidized subunits in which the disulfide bond is formed |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.11.1.24 |
Homo sapiens |
P30044 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.11.1.24 |
H2O2 + reduced thioredoxin |
- |
Homo sapiens |
H2O + oxidized thioredoxin |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.11.1.24 |
peroxiredoxin 5 |
classified in the atypical 2-Cys subfamily, the oxidized form of the enzyme is characterized by the presence of an intramolecular disulfide bridge between the peroxidatic and the resolving cysteine residues |
Homo sapiens |
1.11.1.24 |
PRDX5 |
- |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.11.1.24 |
thioredoxin |
- |
Homo sapiens |
|