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Literature summary extracted from

  • Matsunaga, T.; Endo, S.; Maeda, S.; Ishikura, S.; Tajima, K.; Tanaka, N.; Nakamura, K.T.; Imamura, Y.; Hara, A.
    Characterization of human DHRS4: An inducible short-chain dehydrogenase/reductase enzyme with 3beta-hydroxysteroid dehydrogenase activity (2008), Arch. Biochem. Biophys., 477, 339-347.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.145 T177N significantly improved stability at cold temperature, presence of NAD+ is required for complete stability at 0°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 Cetylpyridinium chloride
-
Homo sapiens
1.1.1.145 genistein
-
Homo sapiens
1.1.1.145 lauric acid 0.05 mM, less than 10% inhibition Homo sapiens
1.1.1.145 myristic acid 0.05 mM, less than 10% inhibition Homo sapiens
1.1.1.145 phenylhexane
-
Homo sapiens
1.1.1.145 quercetin
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0003
-
NADPH pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0003
-
NADP+ pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0015
-
4,4'-dimethylbenzil pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0029
-
5beta-pregnane-3,20-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0036
-
9,10-phenanthrenequinone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0041
-
5beta-androstane-3beta,17beta-diol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0049
-
5beta-androstan-17beta-ol-3-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.005
-
benzil pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0052
-
1-phenylisatin pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0079
-
5beta-androstan-3beta-ol-17-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.008
-
5beta-Pregnan-3beta-ol-20-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.008
-
5beta-pregnane-3beta,20alpha-diol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0084
-
(S)-phenyl-1-butanol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.0097
-
5beta-pregnan-20alpha-ol-3-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.01
-
5beta-androstane-3,17-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.016
-
5alpha-dihydrotestosterone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.024
-
all-trans-retinal pH 7.4, 25°C Homo sapiens
1.1.1.145 0.028
-
5alpha-androstane-3,17-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.035
-
Dehydrolithocholic acid pH 7.4, 25°C Homo sapiens
1.1.1.145 0.036
-
hexanophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.048
-
n-butyrophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.054
-
valerophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.089
-
1,4-Naphthoquinone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.093
-
(S)-1-tetralol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.12
-
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.16
-
4-hexanoylpyridine pH 7.4, 25°C Homo sapiens
1.1.1.145 0.18
-
menadione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.2
-
2,3-heptanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.24
-
(S)-1-indanol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.32
-
isatin pH 7.4, 25°C Homo sapiens
1.1.1.145 0.55
-
2,3-Hexanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 1
-
4-benzoylpyridine pH 7.4, 25°C Homo sapiens
1.1.1.145 1.4
-
dimethyl-2-oxo-glutarate pH 7.4, 25°C Homo sapiens
1.1.1.145 10
-
2,3-Pentanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 13
-
4-nitrobenzaldehyde pH 7.4, 25°C Homo sapiens
1.1.1.145 21
-
Pyridine-4-aldehyde pH 7.4, 25°C Homo sapiens
1.1.1.145 54
-
diacetyl pH 7.4, 25°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
isoform DHRS4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 Hep-G2 cell induction by peroxisome-proliferator-activated receptor alpha ligands Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 (S)-1-indanol + NADP+
-
Homo sapiens ? + NADPH
-
r
1.1.1.145 (S)-1-tetralol + NADP+
-
Homo sapiens ? + NADPH
-
r
1.1.1.145 (S)-phenyl-1-butanol + NADP+
-
Homo sapiens ? + NADPH
-
r
1.1.1.145 1,4-naphthoquinone + NADPH + H+
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 1-phenylisatin + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 2,3-heptanedione + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 2,3-hexanedione + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 2,3-pentanedione + NADPH + H+
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 4,4'-dimethylbenzil + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 4-benzoylpyridine + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 4-hexanoylpyridine + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 4-nitrobenzaldehyde + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 5alpha-androstan-3beta-ol-17-one + NADP+
-
Homo sapiens 5alpha-androstan-3,17-dione + NADPH
-
r
1.1.1.145 5alpha-androstane-3,17-dione + NADPH
-
Homo sapiens 5alpha-androstane-3beta-ol-17-one + NADP+
-
r
1.1.1.145 5alpha-androstane-3beta,17beta-diol + NADP+
-
Homo sapiens 5alpha-androstane-17beta-ol-3-one + NADPH
-
r
1.1.1.145 5alpha-dihydrotestosterone + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 5alpha-pregnane-3beta-ol-20-one + NADP+
-
Homo sapiens 5alpha-pregnane-3,20-dione + NADPH
-
r
1.1.1.145 5beta-androstan-17beta-ol-3-one + NADPH
-
Homo sapiens 5beta-androstane-3beta,17beta-diol + NADP+
-
r
1.1.1.145 5beta-androstan-3beta-ol-17-one + NADP+
-
Homo sapiens 5beta-androstane-3,17-dione + NADPH
-
r
1.1.1.145 5beta-androstane-3,17-dione + NADPH
-
Homo sapiens 5beta-androstan-3beta-ol-17-one + NADP+
-
r
1.1.1.145 5beta-androstane-3beta,17beta-diol + NADP+
-
Homo sapiens 5beta-androstan-17beta-ol-3-one + NADPH
-
r
1.1.1.145 5beta-pregnan-20alpha-ol-3-one + NADPH + H+
-
Homo sapiens 5beta-pregnan-3beta,20alpha-diol + NADP+
-
r
1.1.1.145 5beta-pregnan-3beta-ol-20-one + NADP+
-
Homo sapiens 5beta-pregnan-3,20-dione + NADPH
-
r
1.1.1.145 5beta-pregnane-3,20-dione + NADPH + H+
-
Homo sapiens 5beta-pregnan-3beta-ol-20-one + NADP+
-
r
1.1.1.145 5beta-pregnane-3beta,20alpha-diol + NADP+
-
Homo sapiens 5beta-pregnane-20alpha-ol-3-one + NADPH + H+
-
r
1.1.1.145 6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione + NADPH major product has S-configuration Homo sapiens ? + NADP+
-
r
1.1.1.145 9,10-phenanthrenequinone + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 all-trans-retinal + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 all-trans-retinol + NADP+
-
Homo sapiens ? + NADPH
-
r
1.1.1.145 benzil + NADPH enantiomeric purity of 97% enantiomeric excess Homo sapiens (R)-benzoin + NADP+
-
r
1.1.1.145 dehydrolithocholic acid + NADPH
-
Homo sapiens isolithocholic acid + NADP+
-
r
1.1.1.145 diacetyl + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 dimethyl-2-oxo-glutarate + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 hexanophenone + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 isatin + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 isolithocholic acid + NADP+
-
Homo sapiens dehydrolithocholic acid + NADPH
-
r
1.1.1.145 menadione + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 additional information enzyme exhibits high stereo-selectivity yielding 3-beta-hydroxysteroids from 3-ketosteroids Homo sapiens ?
-
?
1.1.1.145 n-butyrophenone + NADPH major product Homo sapiens (S)-1-phenyl1-butanol + NADP+
-
r
1.1.1.145 pyridine-4-aldehyde + NADPH
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 testosterone + NADPH + H+
-
Homo sapiens ? + NADP+
-
r
1.1.1.145 valerophenone + NADPH
-
Homo sapiens ? + NADP+
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 DHRS4
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.145 0.014
-
5beta-pregnane-3beta,20alpha-diol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.017
-
5beta-androstan-3beta-ol-17-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.017
-
5beta-androstane-3beta,17beta-diol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.041
-
5beta-Pregnan-3beta-ol-20-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.043
-
Dehydrolithocholic acid pH 7.4, 25°C Homo sapiens
1.1.1.145 0.05
-
all-trans-retinal pH 7.4, 25°C Homo sapiens
1.1.1.145 0.063
-
5beta-pregnane-3,20-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.07
-
5beta-pregnan-20alpha-ol-3-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.075
-
(S)-phenyl-1-butanol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.077
-
5beta-androstane-3,17-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.083
-
5beta-androstan-17beta-ol-3-one pH 7.4, 25°C Homo sapiens
1.1.1.145 0.095
-
5alpha-dihydrotestosterone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.097
-
5alpha-androstane-3,17-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.18
-
(S)-1-indanol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.25
-
(S)-1-tetralol pH 7.4, 25°C Homo sapiens
1.1.1.145 0.65
-
menadione pH 7.4, 25°C Homo sapiens
1.1.1.145 0.85
-
n-butyrophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 0.88
-
1,4-Naphthoquinone pH 7.4, 25°C Homo sapiens
1.1.1.145 2
-
4-benzoylpyridine pH 7.4, 25°C Homo sapiens
1.1.1.145 2
-
valerophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 2
-
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione pH 7.4, 25°C Homo sapiens
1.1.1.145 2.7
-
hexanophenone pH 7.4, 25°C Homo sapiens
1.1.1.145 3.7
-
4,4'-dimethylbenzil pH 7.4, 25°C Homo sapiens
1.1.1.145 4.7
-
Pyridine-4-aldehyde pH 7.4, 25°C Homo sapiens
1.1.1.145 11.8
-
9,10-phenanthrenequinone pH 7.4, 25°C Homo sapiens
1.1.1.145 16.3
-
2,3-Hexanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 16.7
-
4-nitrobenzaldehyde pH 7.4, 25°C Homo sapiens
1.1.1.145 26.7
-
2,3-Pentanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 28.3
-
diacetyl pH 7.4, 25°C Homo sapiens
1.1.1.145 31.7
-
isatin pH 7.4, 25°C Homo sapiens
1.1.1.145 31.7
-
benzil pH 7.4, 25°C Homo sapiens
1.1.1.145 33.3
-
4-hexanoylpyridine pH 7.4, 25°C Homo sapiens
1.1.1.145 33.3
-
dimethyl-2-oxo-glutarate pH 7.4, 25°C Homo sapiens
1.1.1.145 40.2
-
2,3-heptanedione pH 7.4, 25°C Homo sapiens
1.1.1.145 41.7
-
1-phenylisatin pH 7.4, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 5.5
-
dimethyl-2-oxoglutarate reductase activity Homo sapiens
1.1.1.145 6.5 10 broad, S-1-tetralol dehydrogenase activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 additional information no significant activity with NAD+/NADH Homo sapiens
1.1.1.145 NADP+
-
Homo sapiens
1.1.1.145 NADPH
-
Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.145 0.0027
-
pH 7.4, 25°C Homo sapiens quercetin
1.1.1.145 0.0059
-
pH 7.4, 25°C Homo sapiens genistein
1.1.1.145 0.0066
-
pH 7.4, 25°C Homo sapiens Cetylpyridinium chloride
1.1.1.145 0.023
-
pH 7.4, 25°C Homo sapiens phenylhexane