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Literature summary extracted from

  • Jawanda, N.; Ebalunode, J.; Gribenko, A.; Briggs, J.; Lee, J.C.; Tu, S.C.
    A single-residue mutation destabilizes Vibrio harveyi flavin reductase FRP dimer (2008), Arch. Biochem. Biophys., 472, 51-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 expressed in Escherichia coli BL21 cells Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.30 E99K the mutation destabilizes the dimer and has an enhanced subunit dissociation as evident from a 44fold higher Kd for the monomer-dimer equilibrium Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.00007
-
 NADPH mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C Vibrio harveyi
1.5.1.30 0.002
-
 FMN mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.30 26300
-
 2 * 26300 Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30 ultracentrifugation, G-25 gel filtration, DEAE-Sepharose column chromatography, and Sephadex G25 gel filtration Vibrio harveyi

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.1.30 soluble denatured mutant enzyme E99K is mixed into 50fold volume of pre-cooled 50 mM phosphate buffer containing 0.1 M FMN and stirred on ice in the dark for 15 min for protein renaturation and the reconstitution of E99K holoenzyme Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 dimer 2 * 26300 Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 FRP
-
 Vibrio harveyi
1.5.1.30 NADPH-specific flavin reductase
-
 Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN contains one bound FMN cofactor per subunit Vibrio harveyi
1.5.1.30 NADPH
-
 Vibrio harveyi