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Literature summary extracted from

  • Fierce, Y.; de Morais Vieira, M.; Piantedosi, R.; Wyss, A.; Blaner, W.S.; Paik, J.
    In vitro and in vivo characterization of retinoid synthesis from beta-carotene (2008), Arch. Biochem. Biophys., 472, 126-138.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.63 type I cellular retinol-binding protein i.e. RBP1, RBP1, but not RBP2, is required for the transport of water-insoluble substrates of retinoid pathway enzymes, e.g. of BCMO1, into the cell, and acts as an intracellular sensor of retinoid status that, when present as apo-RBP1, stimulates BCMO1 activity and the conversion of carotenoids to retinoids Mus musculus
1.13.11.63 type I cellular retinol-binding protein i.e. RBP1, RBP1, but not RBP2, is required for the transport of water-insoluble substrates of retinoid pathway enzymes, e.g. of BCMO1, into the cell, and acts as an intracellular sensor of retinoid status that, when present as apo-RBP1, stimulates BCMO1 activity and the conversion of carotenoids to retinoids Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 EST library screening, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.63 additional information phenotype of enzyme-deficient mice, beta-carotene conversion is blocked in BCMO1-deficient mice, comparison of plasma retinol and liver retinyl esters of wild-type, heterozygous and homozygous BCMO1-deficient mice, overview Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 63000
-
x * 63000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.63 beta-carotene + O2 Mus musculus BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, and is responsible for metabolically limiting the amount of intact beta-carotene that can be absorbed by mice from their diet, regulation of BCMO1 activity, overview all-trans-retinal
-
?
1.13.11.63 beta-carotene + O2 Homo sapiens BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, regulation of BCMO1 activity, overview all-trans-retinal
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Homo sapiens
-
-
-
1.13.11.63 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 beta-carotene + O2
-
Mus musculus all-trans-retinal
-
?
1.13.11.63 beta-carotene + O2
-
Homo sapiens all-trans-retinal
-
?
1.13.11.63 beta-carotene + O2 BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, and is responsible for metabolically limiting the amount of intact beta-carotene that can be absorbed by mice from their diet, regulation of BCMO1 activity, overview Mus musculus all-trans-retinal
-
?
1.13.11.63 beta-carotene + O2 BCMO1 is a key enzyme in the pathway of retinoid synthesis from carotenoids, regulation of BCMO1 activity, overview Homo sapiens all-trans-retinal
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.63 ? x * 63000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 Bcmo1
-
Mus musculus
1.13.11.63 Bcmo1
-
Homo sapiens
1.13.11.63 beta-carotene-15,15'-monooxygenase
-
Mus musculus
1.13.11.63 beta-carotene-15,15'-monooxygenase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.63 37
-
assay at Mus musculus
1.13.11.63 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.63 8
-
assay at Mus musculus
1.13.11.63 8
-
assay at Homo sapiens