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Literature summary extracted from
- Molecular and functional characterization of D-3-phosphoglycerate dehydrogenase in the serine biosynthetic pathway of the hyperthermophilic archaeon Sulfolobus tokodaii (2008), Arch. Biochem. Biophys., 470, 120-128.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | (NH4)2SO4 | enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction | Sulfurisphaera tokodaii |  |
| 1.1.1.95 | K2HPO4 | enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction | Sulfurisphaera tokodaii | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.95 | expressed in Escherichia coli | Sulfurisphaera tokodaii |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.95 | enzyme is highly thermostable | Sulfurisphaera tokodaii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | (NH4)2SO4 | inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 3-phosphohydroxypyruvate | product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: 3-phospho-D-glycerate, inhibition pattern: competitive; product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: NAD, inhibition pattern: non-competitive | Sulfurisphaera tokodaii | |
| 1.1.1.95 | ADP | weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 86% | Sulfurisphaera tokodaii | |
| 1.1.1.95 | AMP | weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 75% | Sulfurisphaera tokodaii | |
| 1.1.1.95 | ATP | weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 85% | Sulfurisphaera tokodaii | |
| 1.1.1.95 | K2HPO4 | inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction. It appears that the phosphate ion PO43- exerts its inhibitory effect by binding to the free enzyme and NADPH-enzyme complex | Sulfurisphaera tokodaii | |
| 1.1.1.95 | additional information | unlike the Escherichia coli PGDH no inhibition by L-serine | Sulfurisphaera tokodaii | |
| 1.1.1.95 | NADH | product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: 3-phospho-D-glycerate, inhibition pattern: non-competitive; product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: NAD, inhibition pattern: competitive | Sulfurisphaera tokodaii | |
| 1.1.1.95 | ZnCl2 | inhibitory effect on 3-phosphohydroxypyruvate reduction, remaining activity: 29% | Sulfurisphaera tokodaii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.95 | 0.0013 | - |
NADH | value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 0.0096 | - |
3-phosphohydroxypyruvate | value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 0.02 | - |
NADPH | value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 0.033 | - |
3-phospho-D-glycerate | value for 3-phospho-D-glycerate oxidation using NAD+ as a cofactor | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 0.04 | - |
NAD+ | value for 3-phospho-D-glycerate oxidation | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 0.12 | - |
3-phosphohydroxypyruvate | value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 35000 | - |
SDS-PAGE | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.95 | Sulfurisphaera tokodaii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.95 | using a butyl Sepharose 4 Fast Flow column and a Superdex 200 gel filtration column. During purification an unexpected decline in enzyme activity is observed if the enzyme is stored in plastic tubes, but not in glass ones | Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | additional information | - |
during purification an unexpected decline in enzyme activity is observed if the enzyme is stored in plastic tubes, but not in glass ones | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.95 | 2-phospho-D-glycerate + NAD+ | activity relative to 3-phospho-D-glycerate: 47% | Sulfurisphaera tokodaii | 2-phosphohydroxypyruvate + NADH + H+ | - |
? | |
| 1.1.1.95 | 3-phospho-D-glycerate + NAD+ | highest activity | Sulfurisphaera tokodaii | 3-phosphohydroxypyruvate + NADH + H+ | - |
r | |
| 1.1.1.95 | 3-phospho-D-glycerate + NADP+ | - |
Sulfurisphaera tokodaii | 3-phosphohydroxypyruvate + NADPH + H+ | - |
r | |
| 1.1.1.95 | DL-glyceraldehyde 3-phosphate + NAD+ | activity relative to 3-phospho-D-glycerate: 9% | Sulfurisphaera tokodaii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.95 | homodimer | gel filtration | Sulfurisphaera tokodaii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.95 | D-3-phosphoglycerate dehydrogenase | - |
Sulfurisphaera tokodaii |
| 1.1.1.95 | PGDH | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 50 | - |
assay at | Sulfurisphaera tokodaii |
| 1.1.1.95 | 53 | - |
optimum temperature for 3-phospho-D-glycerate oxidation | Sulfurisphaera tokodaii |
| 1.1.1.95 | 80 | - |
optimum for 3-phosphohydroxypyruvate reduction | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 50 | 80 | enzyme is highly thermostable, retaining more than 90% of its activity after incubation for 1 h at 80°C | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 7.5 | - |
3-phosphohydroxypyruvate reduction | Sulfurisphaera tokodaii |
| 1.1.1.95 | 10 | - |
3-phospho-D-glycerate oxidation | Sulfurisphaera tokodaii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 3.5 | 11.5 | enzyme is stable over a wide range of pHs. No decrease in activity after 20 min incubation at 50°C | Sulfurisphaera tokodaii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | NAD+ | - |
Sulfurisphaera tokodaii | |
| 1.1.1.95 | NADP+ | NADP utilization is not observed in the forward reaction even in the presence of high concentrations of 10 mM NADP and 10 mM 3-phospho-D-glycerate | Sulfurisphaera tokodaii | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.95 | 2 | - |
K2HPO4 | value of phosphate against 3-phosphohydroxypyruvate | Sulfurisphaera tokodaii | |
| 1.1.1.95 | 10 | - |
K2HPO4 | value of phosphate against NADPH | Sulfurisphaera tokodaii | |
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