EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.81 | DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) | Mentha x piperita |
1.3.1.82 | DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3), expression of a mutant cloning artifact that has a seven residue deletion at the C-terminus and bores an additional 33 vector-derived residues | Mentha x piperita |
EC Number | General Stability | Organism |
---|---|---|
1.3.1.81 | neither protease inhibitors nor flavins improve the activity or stability of the purified native enzyme, and the addition of glycerol to the buffers leads to rapid loss of activity upon storage | Mentha x piperita |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.81 | 0.0023 | - |
(+)-pulegone | pH 5.0, recombinant enzyme | Mentha x piperita | |
1.3.1.81 | 0.0069 | - |
NADPH | pH 5.0, recombinant enzyme | Mentha x piperita | |
1.3.1.82 | 0.001 | - |
(-)-isopiperitenone | pH 5.5, recombinant enzyme | Mentha x piperita | |
1.3.1.82 | 0.0022 | - |
NADPH | pH 5.5, recombinant enzyme | Mentha x piperita |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.81 | 37914 | - |
1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
1.3.1.81 | 43000 | - |
1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
1.3.1.81 | 45000 | - |
gel filtration | Mentha x piperita |
1.3.1.82 | 34409 | - |
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
1.3.1.82 | 38000 | - |
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
1.3.1.82 | 60000 | - |
about, gel filtration | Mentha x piperita |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | Mentha x piperita | pathways of monoterpene biosynthesis, overview | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
1.3.1.82 | (-)-isopiperitenone + NADPH + H+ | Mentha x piperita | pathways of monoterpene biosynthesis, overview | (+)-cis-isopulegone + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.81 | Mentha x piperita | - |
cv. Black Mitcham | - |
1.3.1.82 | Mentha x piperita | - |
cv. Black Mitcham | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.81 | recombinant enzyme from Escherichia coli strain BL21(DE3), native enzyme by oil gland secretory cell isolation procedure, two steps of anion exchange chromatography, hydroxyapatite chromatography, and beta-NADPH affinity chromatography, the activity is rapidly lost during purification on each matrix, 10fold purification accompanied by loss of over 98% of starting activity | Mentha x piperita |
1.3.1.82 | recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) to over 60% homogeneity, and to over 90% purity for the truncated mutant enzyme | Mentha x piperita |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.81 | epidermis | - |
Mentha x piperita | - |
1.3.1.81 | leaf | - |
Mentha x piperita | - |
1.3.1.81 | secretory cell | - |
Mentha x piperita | - |
1.3.1.82 | epidermis | - |
Mentha x piperita | - |
1.3.1.82 | leaf | - |
Mentha x piperita | - |
1.3.1.82 | plant oil gland | - |
Mentha x piperita | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | pathways of monoterpene biosynthesis, overview | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
1.3.1.81 | 2 (+)-pulegone + 2 NADPH + 2 H+ | the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio, product analysis by GC-MS, stereospecific reductase | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
1.3.1.82 | (-)-isopiperitenone + NADPH + H+ | pathways of monoterpene biosynthesis, overview | Mentha x piperita | (+)-cis-isopulegone + NADP+ | - |
? | |
1.3.1.82 | (-)-isopiperitenone + NADPH + H+ | regiospecific and stereospecific reductase | Mentha x piperita | (+)-cis-isopulegone + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.81 | monomer | 1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
1.3.1.82 | dimer | 2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.81 | More | (+)-pulegone reductase is a member of the medium-chain dehydrogenase/reductase superfamily | Mentha x piperita |
1.3.1.81 | pulegone reductase | - |
Mentha x piperita |
1.3.1.82 | More | (-)-isopiperitenone reductase is a member of the short-chain dehydrogenase/reductase superfamily | Mentha x piperita |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.81 | 1.8 | - |
NADPH | pH 5.0, recombinant enzyme | Mentha x piperita | |
1.3.1.81 | 1.8 | - |
(+)-pulegone | pH 5.0, recombinant enzyme | Mentha x piperita | |
1.3.1.82 | 1.3 | - |
NADPH | pH 5.5, recombinant enzyme | Mentha x piperita | |
1.3.1.82 | 1.3 | - |
(-)-isopiperitenone | pH 5.5, recombinant enzyme | Mentha x piperita |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.81 | 5 | - |
- |
Mentha x piperita |
1.3.1.82 | 5.5 | - |
wild-type enzyme | Mentha x piperita |
1.3.1.82 | 6.5 | - |
truncated mutant enzyme | Mentha x piperita |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.81 | 4.5 | 6.5 | 70% of maximal activity within this range | Mentha x piperita |
1.3.1.82 | 4.5 | 7.5 | 50% of maximal activity within this range, wild-type enzyme | Mentha x piperita |
1.3.1.82 | 5 | 8 | 50% of maximal activity within this range, deletion mutant enzyme | Mentha x piperita |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.81 | NADPH | dependent on | Mentha x piperita | |
1.3.1.82 | NADPH | dependent on | Mentha x piperita |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.81 | Mentha x piperita | about, isoelectric focusing | - |
5.2 |