Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kotrbova-Kozak, A.; Kotrba, P.; Inui, M.; Sajdok, J.; Yukawa, H.
    Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R (2007), Appl. Microbiol. Biotechnol., 76, 1347-1356.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Corynebacterium glutamicum R

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.59
-
 propanal 33°C, pH 7 Corynebacterium glutamicum R
1.1.1.1 0.92
-
 acetaldehyde 33°C, pH 7 Corynebacterium glutamicum R
1.1.1.1 2.01
-
 propanone 33°C, pH 7 Corynebacterium glutamicum R
1.1.1.1 3.04
-
 methanol 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 4.24
-
 Butanal 33°C, pH 7 Corynebacterium glutamicum R
1.1.1.1 6.8
-
 ethanol 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 7.49
-
 n-Propanol 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 10.3
-
 n-Pentanal 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 11.9
-
 2-butanol 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 19.6
-
 2-propanol 33°C, pH 8 Corynebacterium glutamicum R
1.1.1.1 35.1
-
 n-butanol 33°C, pH 8 Corynebacterium glutamicum R

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 36900
-
 2 * 36900, SDS-PAGE Corynebacterium glutamicum R
1.1.1.1 81500
-
 gel filtration Corynebacterium glutamicum R

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Corynebacterium glutamicum R
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Corynebacterium glutamicum R

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 culture condition adhA transcription is induced by ethanol or n-propanol, adhA transcription is subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization are detected only after depletion of glucose Corynebacterium glutamicum R
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2-butanol + NAD+
-
 Corynebacterium glutamicum R 2-butanone + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Corynebacterium glutamicum R propanone + NADH + H+
-
 r
1.1.1.1 ethanol + NAD+ no activity with NADP+, in reverse reaction no activity with NADPH Corynebacterium glutamicum R acetaldehyde + NADH + H+
-
 r
1.1.1.1 methanol + NAD+
-
 Corynebacterium glutamicum R formaldehyde + NADH + H+
-
 ?
1.1.1.1 n-butanal + NADH + H+
-
 Corynebacterium glutamicum R n-butanol + NAD+
-
 ?
1.1.1.1 n-butanol + NAD+
-
 Corynebacterium glutamicum R n-butanal + NADH + H+
-
 ?
1.1.1.1 n-pentanol + NAD+
-
 Corynebacterium glutamicum R pentanal + NADH + H+
-
 ?
1.1.1.1 n-propanol + NAD+
-
 Corynebacterium glutamicum R propanal + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 36900, SDS-PAGE Corynebacterium glutamicum R

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 50
-
 reaction with ethanol + NAD+ Corynebacterium glutamicum R

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.7 7.3 reaction with acetaldehyde + NADH Corynebacterium glutamicum R
1.1.1.1 7.7 8.6 reaction with ethanol + NAD+ Corynebacterium glutamicum R