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Literature summary extracted from

  • Liu, Y.J.; Li, P.P.; Zhao, K.X.; Wang, B.J.; Jiang, C.Y.; Drake, H.L.; Liu, S.J.
    Corynebacterium glutamicum contains 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that display novel biochemical features (2008), Appl. Environ. Microbiol., 74, 5497-5503.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA 22.7% residual activity at 2 mM EDTA for isozyme NCgl0950 DAHP synthase, 49.1% residual activity at 2 mM EDTA for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
2.5.1.54 L-Phe NCgl0950 DAHP synthase is sensitive to feedback inhibition with 29.1 to 38.8% inhibition at 2 mM, NCgl2098 DAHP synthase is insensitive Corynebacterium glutamicum
2.5.1.54 L-Trp NCgl0950 DAHP synthase is sensitive to feedback inhibition, NCgl2098 DAHP synthase is slightly sensitive to feedback inhibition with 10-15% decrease of activity at 5 mM L-Trp Corynebacterium glutamicum
2.5.1.54 L-Tyr NCgl0950 DAHP synthase is sensitive to feedback inhibition with 80% loss of activity at 0.03 mM, NCgl2098 DAHP synthase is insensitive, Tyr-linked inhibition is competitive to phophoenolpyruvate Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.16
-
 phosphoenolpyruvate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.5.1.54 0.29
-
 D-erythrose 4-phosphate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.5.1.54 2.17
-
 D-erythrose 4-phosphate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5,at 30°C Corynebacterium glutamicum
2.5.1.54 8.52
-
 phosphoenolpyruvate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ 90.6% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 31.8% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
2.5.1.54 Mn2+ 257.5% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 1343.3% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
2.5.1.54 additional information Mg2+ and Ni2+ are not stimulatory Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 40000
-
 isozyme NCgl0950 DAHP synthase, SDS-PAGE Corynebacterium glutamicum
2.5.1.54 42000
-
 2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum
2.5.1.54 50000
-
 2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration Corynebacterium glutamicum
2.5.1.54 84000
-
 native NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Corynebacterium glutamicum
-
 strains ATCC 13032 and RES167
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 His-Bind protein chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Corynebacterium glutamicum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 homodimer 2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum
2.5.1.54 homodimer 2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 DAHP synthase
-
 Corynebacterium glutamicum
2.5.1.54 NCgl0950 DAHP synthase isozyme Corynebacterium glutamicum
2.5.1.54 NCgl2098 DAHP synthase isozyme Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 0.3
-
 D-erythrose 4-phosphate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.5.1.54 0.35
-
 phosphoenolpyruvate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.5.1.54 1.14
-
 D-erythrose 4-phosphate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.5.1.54 1.65
-
 phosphoenolpyruvate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum