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Literature summary extracted from

  • Borodina, E.; Nichol, T.; Dumont, M.G.; Smith, T.J.; Murrell, J.C.
    Mutagenesis of the "leucine gate" to explore the basis of catalytic versatility in soluble methane monooxygenase (2007), Appl. Environ. Microbiol., 73, 6460-6467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 mutants are expressed in sMMO-negative cells of Methylosinus trichosporium Methylosinus trichosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.25 L110C mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
1.14.13.25 L110G mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
1.14.13.25 L110R mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
1.14.13.25 L110Y mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 biphenyl + NAD(P)H + H+ + O2
-
 Methylosinus trichosporium 2-hydroxybiphenyl + 4-hydroxybiphenyl + NAD(P)+ + H2O
-
 ?
1.14.13.25 ethylbenzene + NAD(P)H + H+ + O2
-
 Methylosinus trichosporium 1-phenylethanol + 3-ethylphenol + 4-ethylphenol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + H+ + O2
-
 Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 additional information inactive toward anthracene and phenanthrene Methylosinus trichosporium ?
-
 ?
1.14.13.25 naphthalene + NAD(P)H + H+ + O2
-
 Methylosinus trichosporium alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
 ?
1.14.13.25 toluene + NAD(P)H + H+ + O2
-
 Methylosinus trichosporium benzyl alcohol + cresol + NAD(P)+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 sMMO
-
 Methylosinus trichosporium
1.14.13.25 soluble methane monooxygenase
-
 Methylosinus trichosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylosinus trichosporium
1.14.13.25 NADPH
-
 Methylosinus trichosporium