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Literature summary extracted from

  • Martinez-Rodriguez, S.; Las Heras-Vazquez, F.J.; Mingorance-Cazorla, L.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.
    Molecular cloning, purification, and biochemical characterization of hydantoin racemase from the legume symbiont Sinorhizobium meliloti CECT 4114 (2004), Appl. Environ. Microbiol., 70, 625-630.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.99.5 expression in Escherichia coli Sinorhizobium meliloti

General Stability

EC Number General Stability Organism
5.1.99.5 the enzyme remains active after 10 freeze-thawing cycles Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.5 3.76
-
 D-isobutylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 6.41
-
 L-isobutylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 8.3
-
 L-benzylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 13.89
-
 D-benzylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 17.32
-
 L-ethylhydantoin pH 8.5 Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.5 31000
-
 4 * 31000, SDS-PAGE Sinorhizobium meliloti
5.1.99.5 100000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.5 Sinorhizobium meliloti
-
-
-
5.1.99.5 Sinorhizobium meliloti CECT 4114
-
-
-

Storage Stability

EC Number Storage Stability Organism
5.1.99.5 -20°C, 0.1 M Tris, 20% glycerol, pH 8.5, stable for at least 3 months Sinorhizobium meliloti
5.1.99.5 4°C, 0.1 M Tris, pH 8.5, stable for 10 weeks Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.5 D-benzylhydantoin
-
 Sinorhizobium meliloti L-benzylhydantoin
-
 r
5.1.99.5 D-benzylhydantoin
-
 Sinorhizobium meliloti CECT 4114 L-benzylhydantoin
-
 r
5.1.99.5 D-isobutylhydantoin
-
 Sinorhizobium meliloti L-isobutylhydantoin
-
 r
5.1.99.5 D-isobutylhydantoin
-
 Sinorhizobium meliloti CECT 4114 L-isobutylhydantoin
-
 r
5.1.99.5 L-benzylhydantoin
-
 Sinorhizobium meliloti D-benzylhydantoin
-
 r
5.1.99.5 L-benzylhydantoin
-
 Sinorhizobium meliloti CECT 4114 D-benzylhydantoin
-
 r
5.1.99.5 L-ethylhydantoin
-
 Sinorhizobium meliloti D-ethylhydantoin
-
 r
5.1.99.5 L-ethylhydantoin
-
 Sinorhizobium meliloti CECT 4114 D-ethylhydantoin
-
 r
5.1.99.5 L-isobutylhydantoin
-
 Sinorhizobium meliloti D-isobutylhydantoin
-
 r
5.1.99.5 L-isobutylhydantoin
-
 Sinorhizobium meliloti CECT 4114 D-isobutylhydantoin
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.99.5 tetramer 4 * 31000, SDS-PAGE Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.99.5 40
-
-
 Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.99.5 1.94
-
 L-benzylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 2.12
-
 L-isobutylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 2.29
-
 D-benzylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 3.24
-
 D-isobutylhydantoin pH 8.5 Sinorhizobium meliloti
5.1.99.5 6.42
-
 L-ethylhydantoin pH 8.5 Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.5 8.5
-
-
 Sinorhizobium meliloti