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Literature summary extracted from

  • Mostafaie, A.; Bidmeshkipour, A.; Shirvani, Z.; Mansouri, K.; Chalabi, M.
    Kiwifruit actinidin: a proper new collagenase for isolation of cells from different tissues (2008), Appl. Biochem. Biotechnol., 144, 123-131.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.14 molecular biology actinidin compared with type II or IV collagenase isolates intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% Actinidia deliciosa
3.4.22.14 synthesis the enzyme can be used efficiently for hydrolysis of collagen and isolation of different cell populations from various solid tissues Actinidia deliciosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia deliciosa
-
-
-
3.4.22.14 Actinidia deliciosa A5HII1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.14
-
 Actinidia deliciosa
3.4.22.14 the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer Actinidia deliciosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.14 fruit
-
 Actinidia deliciosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 Collagen type I + H2O actinidin can hydrolyze collagen types I and II at neutral and alkaline pH Actinidia deliciosa ?
-
 ?
3.4.22.14 Collagen type I + H2O the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate Actinidia deliciosa ?
-
 ?
3.4.22.14 collagen type II + H2O actinidin can hydrolyze collagen types I and II at neutral and alkaline pH Actinidia deliciosa ?
-
 ?
3.4.22.14 collagen type II + H2O the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), or acetate buffer (pH 4), the enzyme does not hydrolyze this substrate Actinidia deliciosa ?
-
 ?
3.4.22.14 additional information actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% Actinidia deliciosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.14 actinidin
-
 Actinidia deliciosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.14 37
-
 assay at Actinidia deliciosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.14 7 8.5 efficient digestion of type I and type II collagen Actinidia deliciosa