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Literature summary extracted from

  • Tosatto, S.C.; Bosello, V.; Fogolari, F.; Mauri, P.; Roveri, A.; Toppo, S.; Flohe, L.; Ursini, F.; Maiorino, M.
    The catalytic site of glutathione peroxidases (2008), Antioxid. Redox Signal., 10, 1515-1526.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.9 expressed in Escherichia coli JM109 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.9 C74A mutation results in a decline of specific activity Drosophila melanogaster
1.11.1.9 N136A mutation results in a dramatic decline of specific activity Drosophila melanogaster
1.11.1.9 N136D mutation results in not detectable specific activity Drosophila melanogaster
1.11.1.9 N136H mutation results in a dramatic decline of specific activity Drosophila melanogaster
1.11.1.9 N40A mutation results in a decline of specific activity Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Drosophila melanogaster Q9VZQ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.9 Ni-NTA resin column chromatography and Superdex 75 gel filtration Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.9 0.24
-
 mutant enzyme N136A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates Drosophila melanogaster
1.11.1.9 0.4
-
 mutant enzyme N136H, using thioredoxin and phosphatidylcholine hydroperoxide as substrates Drosophila melanogaster
1.11.1.9 4.29
-
 mutant enzyme N40A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates Drosophila melanogaster
1.11.1.9 5.81
-
 mutant enzyme C74A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates Drosophila melanogaster
1.11.1.9 14.38
-
 wild type enzyme, using thioredoxin and phosphatidylcholine hydroperoxide as substrates Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 phosphatidylcholine hydroperoxide + thioredoxin
-
 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.9 GPX
-
 Drosophila melanogaster