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Literature summary extracted from
- Development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane (2008), Anal. Chim. Acta, 619, 215-220.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.5.99.13 | analysis | development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane. The D-Pro DH/agar immobilized electrode is applied to determination of D-amino acids in a human urine sample | Pyrobaculum islandicum |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.5.99.13 | enzyme activity in the D-Pro dehydrogenase/agar immobilized electrode is retained over 90% for 80 days | Pyrobaculum islandicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.99.13 | 7.9 | - |
D-proline | - |
Pyrobaculum islandicum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.5.99.13 | membrane | associated to | Pyrobaculum islandicum | 16020 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.99.13 | Pyrobaculum islandicum | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.13 | D-alanine + oxidized 2,6-dichloroindophenol + H2O | 9% of the activity with D-proline | Pyrobaculum islandicum | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-aspartate + oxidized 2,6-dichloroindophenol + H2O | 7% of the activity with D-proline | Pyrobaculum islandicum | oxaloacetate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-histidine + oxidized 2,6-dichloroindophenol + H2O | 29% of the activity with D-proline | Pyrobaculum islandicum | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-isoleucine + oxidized 2,6-dichloroindophenol + H2O | 46% of the activity with D-proline | Pyrobaculum islandicum | 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-leucine + oxidized 2,6-dichloroindophenol + H2O | 19% of the activity with D-proline | Pyrobaculum islandicum | 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-proline + oxidized 2,6-dichloroindophenol | - |
Pyrobaculum islandicum | DELTA1-pyrroline 2-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
| 1.5.99.13 | D-valine + oxidized 2,6-dichloroindophenol + H2O | 18% of the activity with D-proline | Pyrobaculum islandicum | 2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.99.13 | D-Pro DH | - |
Pyrobaculum islandicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.13 | 50 | - |
assay at | Pyrobaculum islandicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.13 | 8 | - |
- |
Pyrobaculum islandicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.13 | FAD | flavoprotein | Pyrobaculum islandicum | |
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Release 2023.1 (January 2023)
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