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Literature summary extracted from

  • Hanson, R.L.; Goldberg, S.L.; Brzozowski, D.B.; Tully, T.P.; Cazzulino, D.; Parker, W.L.; Lyngberg, O.K.; Vu, T.C.; Wong, M.K.; Patel, R.N.
    Preparation of an amino acid intermediate for the dipeptidyl peptidase IV inhibitor, saxagliptin, using a modified phenylalanine dehydrogenase (2007), Adv. Synth. Catal., 349, 1369-1378.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.20 the modified enzyme (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus) is expressed in Pichia pastoris strain SMD1168 or Escherichia coli strain SC 16496 Thermoactinomyces intermedius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 40000
-
 subunit of modified PDH (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus), SDS-PAGE Thermoactinomyces intermedius

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Thermoactinomyces intermedius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20 partial purification by Superdex 200 gel filtration Thermoactinomyces intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + NH3 + NADH modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus is more effective with keto acid 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid and less effective with phenylpyruvate than the wild type PDH Thermoactinomyces intermedius (S)-3-hydroxyadamantylglycine + H2O + NAD+
-
 ?
1.4.1.20 phenylpyruvate + NH3 + NADH
-
 Thermoactinomyces intermedius L-Phe + H2O + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.4.1.20 PDH
-
 Thermoactinomyces intermedius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.20 60
-
 wild type PDH retains most of its activity after 1 h at 60°C whereas modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus loses all activity after 1h at 60°C Thermoactinomyces intermedius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 8.4 9 modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus Thermoactinomyces intermedius

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Thermoactinomyces intermedius