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Literature summary extracted from
- Purification, crystallization and preliminary X-ray analysis of the fumarylacetoacetase family member TTHA0809 from Thermus thermophilus HB8 (2007), Acta Crystallogr. Sect. F, 63, 792-794.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.7.1.2 | medicine | in humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type 1, which is also known as hepatorenal tyrosinaemia | Thermus thermophilus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.7.1.2 | into the vector pET11a for expression in Escherichia coli BL21DE3 cells | Thermus thermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.7.1.2 | a recombinant form of the fumarylacetoacetase family member TTHA0809 from Thermus thermophilus HB8 is crystallized, diffraction data are collected at 2.2 A | Thermus thermophilus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.2 | 29400 | - |
determined by SDS-PAGE | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.2 | 4-fumarylacetoacetate + H2O | Thermus thermophilus | - |
acetoacetate + fumarate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.2 | Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.2 | using a HiPrep 26/10 desalting column, a Super Q Toyopearl 650M column, a Resource Q column, a Bio-Scale CHT-10-I column, and a HiLoad 16/60 Superdex 200 pg column | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.2 | 4-fumarylacetoacetate + H2O | - |
Thermus thermophilus | acetoacetate + fumarate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.2 | FAA | - |
Thermus thermophilus |
| 3.7.1.2 | fumarylacetoacetase | - |
Thermus thermophilus |
| 3.7.1.2 | TTHA0809 | - |
Thermus thermophilus |
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