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Literature summary extracted from

  • Kendziorek, M.; Paszkowski, A.
    Properties of serine:glyoxylate aminotransferase purified from Arabidopsis thaliana leaves (2008), Acta Biochim. Biophys. Sin. (Shanghai), 40, 102-110.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.45 Aminooxyacetate reacts with the carbonyl group of pyridoxal phosphate, 88% inhibition at 0.01 mM Arabidopsis thaliana
2.6.1.45 beta-chloro-L-alanine reacts with the carbonyl group of pyridoxal phosphate. 53.9% inhibition at 1 mM Arabidopsis thaliana
2.6.1.45 p-hydroxymercuribenzoate the 24.1% decrease in enzyme activity achieved using 1.0 mM inhibitor might be considered to be unspecific Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 0.22
-
 pyruvate at 15.4 mM glycine Arabidopsis thaliana
2.6.1.45 0.5
-
 glyoxalate at 30 mM L-alanine Arabidopsis thaliana
2.6.1.45 0.57
-
 Hydroxypyruvate at 15.4 mM glycine Arabidopsis thaliana
2.6.1.45 0.58
-
 L-alanine at 0.5 mM glyoxylate Arabidopsis thaliana
2.6.1.45 0.91
-
 glyoxylate at 30 mM serine Arabidopsis thaliana
2.6.1.45 1.25
-
 glycine at 05 mM pyruvate Arabidopsis thaliana
2.6.1.45 1.25
-
 L-alanine at 10 mM glyoxylate Arabidopsis thaliana
2.6.1.45 1.53
-
 L-serine at 0.5 mM glyoxylate Arabidopsis thaliana
2.6.1.45 2.83
-
 glycine at 0.5 mM hydroxypyruvate Arabidopsis thaliana
2.6.1.45 5
-
 L-serine at 10 mM glyoxylate Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.45 39800
-
 2 * 39800, SDS-PAGE Arabidopsis thaliana
2.6.1.45 82400
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Arabidopsis thaliana Q56YA5 ecotype Landsberg erecta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.45
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.45 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 L-alanine + glyoxylate 55% of the activity with L-serine and glyoxylate Arabidopsis thaliana pyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Arabidopsis thaliana 3-hydroxypyruvate + glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.45 dimer 2 * 39800, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.6.1.45 serine:glyoxylate aminotransferase
-
 Arabidopsis thaliana
2.6.1.45 SGAT
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.45 2.86
-
 L-alanine at 10 mM glyoxylate Arabidopsis thaliana
2.6.1.45 6.4
-
 L-serine at 10 mM glyoxylate Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.45 9.2
-
-
 Arabidopsis thaliana