Literature summary extracted from
Varrelmann, M.; Maiss, E.; Pilot, R.; Palkovics, L.
Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing (2007), J. Gen. Virol., 88, 1005-1015.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.22.45 |
agriculture |
pentapeptide-insertion scanning mutagenesis shown to be a tool for functional characterization of plant virus proteins |
Plum pox virus |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.22.45 |
expressed in Escherichia coli, transformation into Agrobacterium tumefaciens C58C1 by using the binary plant expression vector pBIN61Sa |
Plum pox virus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.22.45 |
additional information |
random-insertion scanning mutagenesis, generation of an pentapeptide-insertion scanning mutagenesis library of helper component protease HC-Pro, performed by using the mutation generation system F701 MGS, pentapeptide scanning mutants in pBIN61S-HC-Pro mapped by restriction digestion, insertion position determined by sequencing |
Plum pox virus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.45 |
Plum pox virus |
P17766 |
genome polyprotein; PPV |
- |
3.4.22.45 |
Plum pox virus PPV |
P17766 |
genome polyprotein; PPV |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.22.45 |
recombinant HC-Pro protein, SDS-PAGE |
Plum pox virus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.4.22.45 |
additional information |
- |
characterization of functional domains of helper component protease HC-Pro involved in RNA-silencing suppression activity determined, systematic analysis of silencing suppression function of helper component protease HC-Pro using pentapeptide-insertion scanning mutagenesis presented, regions with different classes of silencing-suppression activity defined, functional map with information on structural domains, biological functions and mutations affecting silencing-suppressor function or viral synergism indicated |
Plum pox virus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.22.45 |
HC-Pro protein |
- |
Plum pox virus |