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Literature summary extracted from

  • Heiman, M.G.; Engel, A.; Walter, P.
    The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating (2007), J. Cell Biol., 176, 209-222.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.16.6 molecular biology Kex2 and Kex1 may promote cell fusion by proteolytically processing substrates that act in parallel to Prm1 (pheromone-regulated membrane protein 1) as an alternative fusion machine, as cell wall components, or both Saccharomyces cerevisiae
3.4.21.61 molecular biology role of Kex2 during cell fusion. Kex2 may promote cell fusion by proteolytically processing substrates that act in parallel to Prm1 as an alternative fusion machine, as cell wall components, or both Lachancea kluyveri

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.6 additional information lack of the Kex1 protease results in fusion defects during yeast mating Saccharomyces cerevisiae
3.4.21.61 additional information Deltakex2 strongly enhances the cell fusion defect of Prm1-deficient mating pairs and causes a mild fusion defect in otherwise wild-type mating pairs. Deltakex2 and Deltakex1 fusion defects are suppressed by osmotic support. MATalpha/Deltakex2/Deltaprm1 mutant is sterile. A MATa/Deltakex2/Deltaprm1 mutant mates efficiently to a wild-type partner but poorly to a Deltaprm1 partner. Loss of KEX2 in the MATa partner alone decreases fusion by 15% compared with wild-type. Deltakex2 mutation produces a much stronger effect when placed in trans rather than in cis to the Deltaprm1 mutation. Severe cell wall defects in Deltakex2 mutant cells. Deltakex2 mutants produce cytoplasmic blebs embedded in the cell wall Lachancea kluyveri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.61 Golgi apparatus
-
 Lachancea kluyveri 5794
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.6 Saccharomyces cerevisiae
-
-
-
3.4.21.61 Lachancea kluyveri
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.61 Prm2
-
 Lachancea kluyveri ?
-
 ?
3.4.21.61 Ykl077w
-
 Lachancea kluyveri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.6 Kex1
-
 Saccharomyces cerevisiae
3.4.21.61 Kex2
-
 Lachancea kluyveri